Merendino R A, Arena A, Delfino D, Zummo S, Mesiti M, Chillemi S, Bonina L
Institute of Medical Microbiology, University of Messina, Italy.
J Chemother. 1992 Feb;4(1):35-40. doi: 10.1080/1120009x.1992.11739136.
Macrophages, derived from in vitro cultured monocytes from both healthy donors and patients affected by metastatic breast cancer, treated or not with Escherichia coli lipopolysaccharide (LPS), were tested for phagocytosis and intracellular killing of Candida albicans and superoxide anion release. We found a marked impairment in intracellular killing closely linked to the lack of superoxide production in macrophages from patients affected by neoplasia treated or not with LPS. On the other hand, the LPS treatment significantly enhanced the phagocytic activity of all the macrophage populations tested, except for phagocytes obtained from patients affected by neoplasia and differentiated in autologous serum.
对源自健康供体和转移性乳腺癌患者体外培养单核细胞的巨噬细胞进行了测试，这些巨噬细胞无论是否用大肠杆菌脂多糖（LPS）处理，均检测其对白色念珠菌的吞噬作用、细胞内杀伤能力以及超氧阴离子释放情况。我们发现，无论是否用LPS处理，肿瘤患者巨噬细胞的细胞内杀伤能力均显著受损，这与超氧阴离子产生不足密切相关。另一方面，LPS处理显著增强了所有测试巨噬细胞群体的吞噬活性，但从肿瘤患者获得并在自体血清中分化的吞噬细胞除外。
