Tugwood J D, Lau W H, O S K, Tsao S Y, Martin W M, Shiu W, Desgranges C, Jones P H, Arrand J R
J Gen Virol. 1987 Apr;68 ( Pt 4):1081-91. doi: 10.1099/0022-1317-68-4-1081.
Cytoplasmic RNA was prepared from biopsy material obtained from the nasopharynx of normal individuals and nasopharyngeal carcinoma (NPC) patients. Similar RNA preparations were prepared from lymphocytes of healthy donors and infectious mononucleosis patients. RNA was radioactively labelled in vitro and hybridized to cloned fragments of B95-8 Epstein-Barr virus (EBV). In all seropositive cases the minimum pattern of EBV-specific RNA expression was like that observed previously in latently infected, EBV-positive lymphoblastoid cell lines or Burkitt's lymphoma biopsies. A novel observation was that all of the control biopsies from normal nasopharynxes expressed EBV-specific RNA, some of which appeared to be associated with a more active state of EBV infection. The pattern of expression in NPC patients was similar to that of the normal donors but showed some variations in complexity. In general the virus-specified small RNAs were not present in normal nasopharyngeal tissue but were expressed in the NPC biopsies.
从正常个体和鼻咽癌（NPC）患者的鼻咽部活检材料中制备细胞质RNA。从健康供体和传染性单核细胞增多症患者的淋巴细胞中制备类似的RNA制剂。RNA在体外进行放射性标记，并与B95-8 Epstein-Barr病毒（EBV）的克隆片段杂交。在所有血清阳性病例中，EBV特异性RNA表达的最小模式与先前在潜伏感染的EBV阳性淋巴母细胞系或伯基特淋巴瘤活检中观察到的模式相似。一个新的观察结果是，所有来自正常鼻咽部的对照活检都表达EBV特异性RNA，其中一些似乎与EBV感染的更活跃状态有关。NPC患者的表达模式与正常供体相似，但在复杂性上有一些差异。一般来说，病毒特异性小RNA在正常鼻咽组织中不存在，但在NPC活检中表达。
