Niedobitek G, Young L S, Sam C K, Brooks L, Prasad U, Rickinson A B
Department of Cancer Studies, University of Birmingham, United Kingdom.
Am J Pathol. 1992 Apr;140(4):879-87.
Previous studies investigating the role of Epstein-Barr virus (EBV) in undifferentiated nasopharyngeal carcinoma (NPC) have been performed on extracts from biopsies. The authors analyzed expression and localization of viral gene products in 18 undifferentiated NPCs at the cellular level using immunohistology and in situ hybridization. All cases were EBV-positive. The small nuclear EBV-encoded RNAs, EBERs, were regularly expressed whereas the latent membrane protein, LMP1, and EBV was detectable only in four cases (22%) and the nuclear antigen 2 was not detectable. The BZLF-1 protein of EBV which disrupts viral latency, was not detectable, confirming that the virus is latent in the tumor cells. Although the expression of the CD23 antigen in transplantable NPCs has been reported, our study demonstrates that expression of this antigen in human undifferentiated NPCs is rare. In contrast, almost all cases expressed the CDw70 antigen. Since in normal tissues this antigen is present only in activated lymphoid blasts, this finding may be relevant for the differential diagnosis of undifferentiated NPCs.
以往关于爱泼斯坦-巴尔病毒（EBV）在未分化鼻咽癌（NPC）中作用的研究是在活检组织提取物上进行的。作者使用免疫组织学和原位杂交技术，在细胞水平分析了18例未分化鼻咽癌中病毒基因产物的表达和定位。所有病例均为EBV阳性。小核EBV编码RNA（EBERs）有规律地表达，而潜伏膜蛋白（LMP1）仅在4例（22%）中可检测到，EBV仅在4例（22%）中可检测到，核抗原2未检测到。EBV的BZLF-1蛋白可破坏病毒潜伏状态，未检测到，证实该病毒在肿瘤细胞中处于潜伏状态。虽然已有报道可移植性鼻咽癌中CD23抗原的表达，但我们的研究表明，该抗原在人类未分化鼻咽癌中的表达很少见。相反，几乎所有病例都表达CDw70抗原。由于在正常组织中该抗原仅存在于活化的淋巴母细胞中，这一发现可能与未分化鼻咽癌的鉴别诊断有关。
