R&D Abbott Nutrition, 18004 Granada, Spain.
Doctoral programme in Biomedicine, School of Health Sciences, University of Granada, 18071 Granada, Spain.
Nutrients. 2018 Oct 16;10(10):1519. doi: 10.3390/nu10101519.
Sialic acids (Sia) are postulated to improve cognitive abilities. This study evaluated Sia effects on rat behavior when administered in a free form as N-acetylneuraminic acid (Neu5Ac) or conjugated as 6'-sialyllactose (6'-SL). Rat milk contains Sia, which peaks at Postnatal Day 9 and drops to a minimum by Day 15. To bypass this Sia peak, a cohort of foster mothers was used to raise the experimental pups. A group of pups received a daily oral supplementation of Neu5Ac to mimic the amount naturally present in rat milk, and another group received the same molar amount of Sia as 6'-SL. The control group received water. After weaning, rats were submitted to behavioral evaluation. One year later, behavior was re-evaluated, and in vivo long-term potentiation (LTP) was performed. Brain samples were collected and analyzed at both ages. Adult rats who received Sia performed significantly better in the behavioral assessment and showed an enhanced LTP compared to controls. Within Sia groups, 6'-SL rats showed better scores in some cognitive outcomes compared to Neu5Ac rats. At weaning, an effect on polysialylated-neural cell adhesion molecule (PSA-NCAM) levels in the frontal cortex was only observed in 6'-SL fed rats. Providing Sia during lactation, especially as 6'-SL, improves memory and LTP in adult rats.
唾液酸（Sia）被认为可以改善认知能力。本研究评估了 Sia 以游离形式作为 N-乙酰神经氨酸（Neu5Ac）或作为 6'-唾液酸乳糖（6'-SL）给药时对大鼠行为的影响。大鼠乳中含有 Sia，其在产后第 9 天达到峰值，到第 15 天降至最低。为了避免这种 Sia 峰值，使用了一批寄养母亲来喂养实验幼崽。一组幼崽每天接受 Neu5Ac 的口服补充，以模拟大鼠乳中天然存在的量，另一组接受与 6'-SL 相同摩尔量的 Sia。对照组给予水。断奶后，对大鼠进行行为评估。一年后，重新进行行为评估，并进行体内长时程增强（LTP）。在两个年龄阶段收集和分析脑样本。接受 Sia 的成年大鼠在行为评估中表现明显更好，与对照组相比，LTP 增强。在 Sia 组中，与 Neu5Ac 大鼠相比，6'-SL 大鼠在一些认知结果中表现出更好的分数。在断奶时，仅在 6'-SL 喂养的大鼠中观察到额皮质中多唾液酸化神经细胞粘附分子（PSA-NCAM）水平的变化。在哺乳期提供 Sia，特别是作为 6'-SL，可以改善成年大鼠的记忆和 LTP。
