Vazquez Enrique, Barranco Alejandro, Ramirez Maria, Gruart Agnes, Delgado-Garcia Jose M, Jimenez Maria L, Buck Rachael, Rueda Ricardo
Strategic R&D Department, Abbott Nutrition, Granada, 18004, Spain.
Division of Neurosciences, Pablo de Olavide University, Seville, 41013, Spain.
PLoS One. 2016 Nov 16;11(11):e0166070. doi: 10.1371/journal.pone.0166070. eCollection 2016.
2´-fucosyllactose (2´-FL) is an abundant human milk oligosaccharide (HMO) in human milk with diverse biological effects. We recently reported ingested 2´-FL stimulates central nervous system (CNS) function, such as hippocampal long term potentiation (LTP) and learning and memory in rats. Conceivably the effect of 2´-FL on CNS function may be via the gut-brain axis (GBA), specifically the vagus nerve, and L-fucose (Fuc) may play a role. This study had two aims: (1) determine if the effect of ingested 2´-FL on the modulation of CNS function is dependent on the integrity of the molecule; and (2) confirm if oral 2´-FL modified hippocampal LTP and associative learning related skills in rats submitted to bilateral subdiaphragmatic vagotomy. Results showed that 2´-FL but not Fuc enhanced LTP, and vagotomy inhibited the effects of oral 2´-FL on LTP and associative learning related paradigms. Taken together, the data show that dietary 2´-FL but not its Fuc moiety affects cognitive domains and improves learning and memory in rats. This effect is dependent on vagus nerve integrity, suggesting GBA plays a role in 2´-FL-mediated cognitive benefits.
2'-岩藻糖基乳糖（2'-FL）是母乳中一种含量丰富的人乳寡糖（HMO），具有多种生物学效应。我们最近报道，摄入的2'-FL可刺激中枢神经系统（CNS）功能，如大鼠海马体的长时程增强（LTP）以及学习和记忆能力。可以想象，2'-FL对CNS功能的影响可能是通过肠-脑轴（GBA），特别是迷走神经，并且L-岩藻糖（Fuc）可能发挥作用。本研究有两个目的：（1）确定摄入的2'-FL对CNS功能调节的影响是否依赖于分子的完整性；（2）证实口服2'-FL是否能改变双侧膈下迷走神经切断术后大鼠的海马体LTP和关联学习相关技能。结果表明，2'-FL而非Fuc增强了LTP，并且迷走神经切断术抑制了口服2'-FL对LTP和关联学习相关范式的影响。综上所述，数据表明饮食中的2'-FL而非其Fuc部分会影响大鼠的认知领域并改善学习和记忆。这种效应依赖于迷走神经的完整性，表明GBA在2'-FL介导的认知益处中发挥作用。
