Department of Medical Parasitology, School of Basic Medical Sciences, Central South University, No. 172, Tongzipo Road, Changsha, 410013, Hunan, People's Republic of China.
Laboratory of Molecular Biology and Genetics (LMBG) (Labiogene), University Joseph KI-ZERBO, CERBA/LABIOGENE, 01, BP 364, Ouagadougou 01, Burkina Faso.
Sci Rep. 2024 Mar 28;14(1):7369. doi: 10.1038/s41598-024-58179-4.
Tandem repeat genetic profiles used in forensic applications varies between populations. Despite the diversity and security issues in the Sahel that require the identification of victims (soldiers and civilians), Burkina Faso (BF) remains understudied. To fill this information gap, 396 unrelated individuals from BF were genotyped using a MICROREADER 21 ID System kit. All 20 short tandem repeat (STR) loci tested passed the Hardy-Weinberg equilibrium (HWE) test. The combined powers of exclusion for duos (CPE duos) and trios (CPE trios) for the 20 tested loci were 0.9999998 and 0.9999307, respectively. The probability that two individuals would share the same DNA profiles among the BF population was 9.80898 × 10. For the X-chromosome STR analysis, 292 individuals were included in this study using a MICROREADER 19X Direct ID System kit. Among the 19 loci, no significant deviations from HWE test were observed in female samples after Bonferroni correction (p < 0.05/19 = 0.0026), except for loci GATA165B12 and DXS7423. The results showed that the combined power of exclusion (CPE) and the combined power of discrimination in females (CPDF) and males (CPDM) were 0.999999760893, 0.999999999992, and 1, respectively. Comparison with other African sub-populations showed that geographical proximity is a reliable indicator of genetic relatedness.
法医学应用中使用的串联重复基因谱在不同人群之间存在差异。尽管萨赫勒地区存在多样性和安全问题，需要对受害者（士兵和平民）进行身份识别，但布基纳法索（BF）的相关研究仍然较少。为了填补这一信息空白，对来自 BF 的 396 名无关个体使用 MICROREADER 21 ID 系统试剂盒进行了基因分型。所有 20 个短串联重复（STR）基因座均通过 Hardy-Weinberg 平衡（HWE）测试。对于 20 个测试基因座的双人（CPE 双人）和三人（CPE 三人）排除能力（CPE）分别为 0.9999998 和 0.9999307。BF 人群中两个个体具有相同 DNA 谱的概率为 9.80898×10-9。对于 X 染色体 STR 分析，本研究使用 MICROREADER 19X Direct ID 系统试剂盒纳入了 292 名个体。在 19 个基因座中，女性样本在经过 Bonferroni 校正后（p<0.05/19=0.0026），除 GATA165B12 和 DXS7423 基因座外，其余基因座均未偏离 HWE 测试。结果表明，女性的排除能力总和（CPE）和女性（CPDF）和男性（CPDM）的鉴别能力总和分别为 0.999999760893、0.999999999992 和 1。与其他非洲亚人群的比较表明，地理位置的接近是遗传相关性的可靠指标。
