Kapsokalyvas Dimitrios, Wilbers Arnold, Boogers Ilco A L A, Appeldoorn Maaike M, Kabel Mirjam A, Loos Joachim, Van Zandvoort Marc A M J
1Department of Molecular Cell Biology,CARIM,GROW,Maastricht University,Maastricht,Universiteitssingel 50,6229 ER Maastricht,The Netherlands.
2Royal DSM N.V., Materials Science Center,Urmonderbaan 22,Geleen6167 RD,The Netherlands.
Microsc Microanal. 2018 Oct;24(5):517-525. doi: 10.1017/S1431927618015143.
Parameters such as pretreatment method, enzyme type and concentration, determine the conversion efficiency of biomass' cellulose and hemicellulose to glucose and mainly xylose in biomass-based fuel production. Chemical quantification of these processes offers no information on the effect of enzymatic hydrolysis (EH) on particle morphology. We report on the development of a microscopy method for imaging pretreated biomass particles at different EH stages. The method was based on acquiring large field of view images, typically 20×10 mm2 containing thousands of particles. Morphology of particles with lengths between 2 μm and 5 mm could be visualized and analyzed. The particle length distribution of corn stover samples, pretreated with increasing amounts of sulfuric acid at different EH stages, was measured. Particle size was shown to be dependent on pretreatment severity and EH time. The methodology developed could offer an alternative method for characterization of EH of biomass for second generation biofuels and visualization of recalcitrant structures.
诸如预处理方法、酶的类型和浓度等参数，决定了在基于生物质的燃料生产中，生物质的纤维素和半纤维素转化为葡萄糖以及主要是木糖的转化效率。这些过程的化学定量分析无法提供有关酶水解（EH）对颗粒形态影响的信息。我们报告了一种用于在不同EH阶段对预处理生物质颗粒进行成像的显微镜方法的开发。该方法基于获取大视野图像，通常为20×10平方毫米，包含数千个颗粒。长度在2微米至5毫米之间的颗粒形态可以可视化并进行分析。测量了在不同EH阶段用不同量硫酸预处理的玉米秸秆样品的颗粒长度分布。结果表明颗粒大小取决于预处理的强度和EH时间。所开发的方法可为第二代生物燃料生物质的EH表征和顽固结构的可视化提供一种替代方法。
