Li Dak Sum Yip Yio Chin R&D Centre for Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China.
Shiu-Ying Hu Herbarium, The Chinese University of Hong Kong, Hong Kong, China.
Database (Oxford). 2018 Jan 1;2018:bay112. doi: 10.1093/database/bay112.
Authentication of medicinal materials by deoxyribonucleic acid (DNA) technology is gaining popularity. In 2010, our team has created Medicinal Materials DNA Barcode Database (MMDBD) version 1.0 to provide an interactive database for documenting DNA barcode sequences of medicinal materials. This database now contains DNA barcode sequences of medicinal materials listed in the Chinese Pharmacopoeia, Dietary Supplements Compendium and Herbal Medicine Compendium of the US Pharmacopoeia and selected adulterants. The data archive is regularly updated and currently it stores 62 011 DNA sequences of 2111 medicinal materials. Our team has recently completed the major improvement on the interfaces and incorporated essential bioinformatics tools to facilitate the authentication work. MMDBD version 1.5 contains detailed information of each medicinal material including their material names, medical part, pharmacopeia information, biological classification in rank of family and status on the Convention on International Trade in Endangered Species of Wild Fauna and Flora and the International Union for Conservation of Nature's Red List of Threatened Species, if any. DNA sequences can be retrieved by search in Latin scientific name, Chinese name, family name, material name, medical part and simplified Chinese character stroke. A BLAST'-based engine for searching DNA sequences is included in the MMDBD version 1.5. Since primer design is a key step in DNA barcoding authentication, we have integrated the Clustal Omega alignment tool' and `Primer3' in the form of web interface. These new tools facilitate multiple sequence comparison and the design of primers for amplification of a target DNA barcode region, allowing DNA barcoding authentication.
基于脱氧核糖核酸（DNA）技术的中药材鉴定正日益普及。2010 年，我们的团队创建了中药材 DNA 条形码数据库（MMDBD）版本 1.0，为中药材 DNA 条形码序列提供了一个交互式数据库。该数据库现在包含了《中国药典》、《美国药典膳食补充剂专论》和《草药专论》中列出的中药材以及一些选定的掺杂物的 DNA 条形码序列。数据档案定期更新，目前存储了 2111 种中药材的 62011 条 DNA 序列。我们的团队最近完成了界面的重大改进，并整合了必要的生物信息学工具，以促进鉴定工作。MMDBD 版本 1.5 包含了每种中药材的详细信息，包括其物质名称、药用部位、药典信息、在生物分类中的科级分类以及在《濒危野生动植物种国际贸易公约》和《国际自然保护联盟濒危物种红色名录》中的地位（如有）。可以通过拉丁学名、中文名、科名、物质名、药用部位和简体中文字符笔画搜索来检索 DNA 序列。一个基于 BLAST 的 DNA 序列搜索引擎也包含在 MMDBD 版本 1.5 中。由于引物设计是 DNA 条形码鉴定的关键步骤，我们将“Clustal Omega 比对工具”和“Primer3”集成到了网页界面中。这些新工具便于进行多序列比较和目标 DNA 条形码区域的引物设计，从而实现 DNA 条形码鉴定。
