Storm J F
Neurosci Lett. 1987 Mar 20;75(1):71-4. doi: 10.1016/0304-3940(87)90077-2.
Intracellular recordings were made from CA1 pyramidal cells in rat hippocampal slices. Single action potentials were elicited by injection of brief current pulses. Bath application of phorbol esters (4 beta-phorbol-12,13-diacetate, 0.3-5 microM; or 4 beta-phorbol-12,13-dibutyrate, 5-10 microM) broadened the action potential in each of the cells tested (n = 9). The broadening reflected slowing of the repolarization, whereas the upstroke of the spike was unchanged. This effect may enhance transmitter release from synaptic terminals, and contribute to enhancement of synaptic transmission through activation of protein kinase C, a mechanism which has been associated with long term potentiation.
在大鼠海马脑片的CA1锥体细胞上进行了细胞内记录。通过注入短暂的电流脉冲来诱发单个动作电位。浴槽应用佛波酯（4β-佛波醇-12,13-二乙酸酯，0.3 - 5微摩尔；或4β-佛波醇-12,13-二丁酸酯，5 - 10微摩尔）使所测试的每个细胞（n = 9）的动作电位增宽。这种增宽反映了复极化的减慢，而动作电位的上升支未改变。这种效应可能增强突触终末的递质释放，并通过激活蛋白激酶C促进突触传递的增强，这一机制与长时程增强有关。
