Riahi Leila, Chakroun Hanen, Klay Imen, Masmoudi Ahmed Slaheddine, Cherif Ameur, Zoghlami Nejia
Laboratory of Biotechnology and Bio-Geo Resources Valorization BVBGR-LR11ES31, Higher Institute of Biotechnology of Sidi Thabet, University of Manouba, 2020, Sidi Thabet, Ariana, Tunisia.
Laboratory of Plant Molecular Physiology, Centre of Biotechnology of Borj Cedria, B.P. 901, Hammam-Lif 2050, Tunisia.
J Complement Integr Med. 2018 Oct 17;16(2):/j/jcim.2019.16.issue-2/jcim-2018-0048/jcim-2018-0048.xml. doi: 10.1515/jcim-2018-0048.
Background The Mentha rotundifolia L. (Lamiaceae family), is a medicinal herb used since the ancient times as an antiseptic, analgesic and anti-inflammatory agent. In the present work, metabolomic profiling of two Mentha rotundifolia L. ecotypes leaf tissues spontaneously growing in the North of Tunisia was achieved. Methods Phenolic contents (TPC, TFC and TTC) were assessed using colorimetric methods. Metabolomic profiling of leaf tissues extracts was assessed based on Gas Chromatography-Mass Spectrometry (GC/MS) analysis. The antioxidant ability of M. rotundifolia extracts was achieved based on two test systems namely DPPH and FRAP assays. Antimicrobial activity against a set of Gram negative and Gram positive bacteria was estimated by measuring ID, MIC and MBC values. Results Fifty metabolites were identified as belonging mainly to phenolics, fatty acids, terpenes, steroids and aldehydes classes with qualitative and quantitative variability. Most of the identified compounds are reputed bioactive with potent antioxidant, antimicrobial and anti-inflammatory among others effects. To confirm these findings common in vitro biological activities were achieved. The investigated extracts showed significant antioxidant abilities based on both 1,1-diphenyl-2-picrylhydrazyl (DPPH) and Ferric reducing antioxidant potential (FRAP) assays. Furthermore, the extracts revealed promising antimicrobial ability against tested Gram+ and Gram- bacterial strains (ID: 12.5-14.5 mm, MIC: 3.125-25 (µg/mL), MBC: 6.25-100 (µg/mL)). Conclusions Based on our findings Mentha rotundifolia L. leaves extracts present a potential source of natural antioxidants and diverse bioactive compounds which could be used in green pharmacy, food preservation, alternative medicine and natural therapies.
背景 圆叶薄荷（唇形科）是一种自古以来就被用作防腐剂、止痛剂和抗炎剂的药草。在本研究中，对突尼斯北部自然生长的两种圆叶薄荷生态型叶片组织进行了代谢组学分析。方法 采用比色法评估酚类含量（总酚含量、总黄酮含量和总单宁含量）。基于气相色谱-质谱联用（GC/MS）分析对叶片组织提取物进行代谢组学分析。基于二苯基苦味酰基自由基（DPPH）和铁离子还原抗氧化能力（FRAP）两种测试系统测定圆叶薄荷提取物的抗氧化能力。通过测量抑菌圈直径（ID）、最低抑菌浓度（MIC）和最低杀菌浓度（MBC）值评估对一组革兰氏阴性菌和革兰氏阳性菌的抗菌活性。结果 鉴定出50种代谢物，主要属于酚类、脂肪酸、萜类、类固醇和醛类，存在定性和定量差异。大多数已鉴定的化合物被认为具有生物活性，具有强大的抗氧化、抗菌和抗炎等作用。为证实这些常见发现，进行了体外生物学活性研究。基于1,1-二苯基-2-苦基肼基（DPPH）和铁离子还原抗氧化能力（FRAP）测定，所研究的提取物显示出显著的抗氧化能力。此外，提取物对测试的革兰氏阳性菌和革兰氏阴性菌菌株显示出有前景的抗菌能力（抑菌圈直径：12.5 - 14.5毫米，最低抑菌浓度：3.125 - 25（微克/毫升），最低杀菌浓度：6.25 - 100（微克/毫升））。结论 根据我们的研究结果，圆叶薄荷叶片提取物是天然抗氧化剂和多种生物活性化合物的潜在来源，可用于绿色制药、食品保鲜、替代医学和自然疗法。
