(L.) Huds. aqueous extract attenuates HO induced oxidative stress and neurotoxicity.

INTRODUCTION

Oxidative stress plays a causal role in neurodegenerative diseases. The aim of this study is to evaluate the antioxidant and neuroprotective effects of (L.) Huds (), a widely used Moroccan plant in traditional medicine.

METHODS

The chemical composition of aqueous extract was analyzed by liquid chromatography coupled to mass spectrometry (LC-MS). 2,2-diphenyl 1-picrylhydrazyl (DPPH) and 2,2'-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assays were used to assess its antioxidant activity. HO was utilized to induce oxidative stress and neurotoxicity . Behavioral changes were evaluated using Open Field, Y-maze and Rotarod tests. Hyperalgesia was assessed using the tail immersion test.

RESULTS AND DISCUSSION

The LC-MS/MS analysis revealed high content of kaempferol glucuronide (85%) at the extract. IC values of the DPPH and ABTS were 26.47 and 41.21 μg/mL, respectively. Pre-treatments with extract attenuated the behavioral changes induced by HO. In addition, the latency of tail withdrawal increased significantly in the treated groups suggesting central analgesic effect of extract. Moreover, the extract attenuated the deleterious effects of HO and improved all liver biomarkers. The obtained results suggested that had remarkable antioxidant and neuroprotective effects and may prevent oxidative stress related disorders.