Guangdong Province Engineering Research Center for Urban Water Recycling and Environmental Safety, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.
State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.
Bioresour Technol. 2019 Jan;272:146-155. doi: 10.1016/j.biortech.2018.10.017. Epub 2018 Oct 6.
Nitrogen removal and microbial interactions in two combined nitritation and Anammox systems with or without the addition of organics were examined. Two systems were successfully started up by adopting intermittent aeration. Organics addition deteriorated nitrogen removal, and total inorganic nitrogen and ammonium removal percentages decreased by 16.4% and 26.3%, respectively. Organics addition promoted the growth of Chloroflexi and Proteobacteria while suppressed the growth of Candidatus Kuenenia. Organics addition decreased activities of fatty acid biosynthesis and metabolism, amino acid metabolism and biofilm formation, while increased activities of steroid metabolism and glycosaminoglycan biosynthesis. Heterotrophs and Candidatus Kuenenia might interact with other organisms by using diverse quorum sensing systems. Chloroflexi and Proteobacteria interacted with Candidatus Kuenenia in nitrogen metabolism and biofilm formation. Proteobacteria played a key role in building a nitrite loop with Candidatus Kuenenia and nitrifiers. These results clarified microbial interactions in the autotrophic nitrogen removal process and advance its application.
考察了在添加或不添加有机物的两种组合式亚硝化和厌氧氨氧化系统中氮的去除和微生物相互作用。通过间歇曝气成功地启动了两个系统。有机物的添加恶化了氮的去除，总无机氮和氨氮的去除率分别下降了 16.4%和 26.3%。有机物的添加促进了绿弯菌门和变形菌门的生长，而抑制了卡氏菌属的生长。有机物的添加降低了脂肪酸生物合成和代谢、氨基酸代谢和生物膜形成的活性，而增加了类固醇代谢和糖胺聚糖生物合成的活性。异养菌和卡氏菌属可能通过使用不同的群体感应系统与其他生物相互作用。绿弯菌门和变形菌门在氮代谢和生物膜形成方面与卡氏菌属相互作用。变形菌门在与卡氏菌属和硝化菌建立亚硝酸盐循环方面发挥了关键作用。这些结果阐明了自养脱氮过程中的微生物相互作用，并推进了其应用。
