School of Pharmaceutical Sciences, Shandong University, 44 West Wenhua Road, Jinan 250012, Shandong, China.
Department of Pathology and Pathophysiology, School of Basic Medical Sciences, Shandong University, 44 West Wenhua Road, Jinan 250012, China.
Molecules. 2018 Oct 17;23(10):2668. doi: 10.3390/molecules23102668.
In this study, the aim was to investigate the effect of bergenin on immune function and antioxidation in cyclophosphamide (Cy)-induced immunosuppressed mice. Firstly, we estimated its effect on immune organs. Histological analysis and indexes of immune organs showed that cyclophosphamide exhibited spleen and thymus injury compared with the normal control, which was alleviated by bergenin. Secondly, bergenin also enhanced the humoral immune function through increasing the level of IgM and IgG in serum. Thirdly, bergenin also enhanced the cellular immune function. The results indicate that bergenin increased peritoneal macrophage functions, the proliferation of T and B lymphocytes, NK and CTL cell activities, and T (CD4⁺ and CD8⁺) lymphocyte subsets. Besides, bergenin also had the ability to modulate the Th1/Th2 balance. Moreover, bergenin prevented the Cy-induced decrease in numbers of peripheral RBC, WBC and platelets, providing supportive evidence for their anti-leukopenia activities. Finally, bergenin also reversed the Cy-induced decrease in the total antioxidant capacity including activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px). In conclusion, bergenin protected against Cy-induced adverse reactions by enhancing humoral and cellular immune functions and augmenting antioxidative activity and could be considered as a potential immunomodulatory agent.
在这项研究中，目的是研究小檗因对环磷酰胺（Cy）诱导免疫抑制小鼠免疫功能和抗氧化作用的影响。首先，我们评估了它对免疫器官的影响。免疫器官的组织学分析和指标表明，与正常对照组相比，环磷酰胺表现出脾脏和胸腺损伤，而小檗因减轻了这种损伤。其次，小檗因通过增加血清中 IgM 和 IgG 的水平也增强了体液免疫功能。第三，小檗因也增强了细胞免疫功能。结果表明，小檗因增加了腹腔巨噬细胞的功能、T 和 B 淋巴细胞的增殖、NK 和 CTL 细胞的活性以及 T（CD4⁺和 CD8⁺）淋巴细胞亚群。此外，小檗因还具有调节 Th1/Th2 平衡的能力。此外，小檗因还防止了 Cy 引起的外周 RBC、WBC 和血小板数量减少，为其抗白细胞减少活性提供了支持证据。最后，小檗因还逆转了 Cy 诱导的总抗氧化能力的降低，包括超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）的活性。总之，小檗因通过增强体液和细胞免疫功能以及增强抗氧化活性来防止 Cy 诱导的不良反应，可被认为是一种潜在的免疫调节剂。
