School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, Shandong, China.
Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao 266003, Shandong, China.
Mar Drugs. 2017 Nov 7;15(11):347. doi: 10.3390/md15110347.
In this study, the immunomodulatory activities of glycosaminoglycan (AHG) on the nature killer (NK) cells, cytotoxic T lymphocytes (CTLs) and cyclophosphamide (CY)-treated mice were investigated. After stimulation with multiple concentrations of AHG (0-100 μg/mL), NK cells and CTLs displayed outperformance against YAC-1 and B16 cells, respectively. Furthermore, the mitogen-induced splenic lymphocyte proliferation in CY-induced immunosuppressed mice was significantly promoted by AHG. In addition, the administration of AHG dramatically increased the splenocytes Ca concentration and the delayed-type hypersensitivity (DTH) reaction in a dose-dependent manner. Besides, AHG could strongly increase the total antioxidant capacity (T-AOC), the activities of superoxidase dismutase (SOD), catalase (CAT) as well as glutathione peroxidase (GSH-PX), and could decrease the malondialdehyde (MDA) level in the heart, kidney and liver. These findings indicated that AHG played an important role in the immune enhancement and protection against CY-induced immunosuppression and oxidative damage. Our findings provide experimental evidence for further research and possible immunostimulatory applications of AHG in clinical practice.
在这项研究中，研究了糖胺聚糖（AHG）对自然杀伤（NK）细胞、细胞毒性 T 淋巴细胞（CTL）和环磷酰胺（CY）处理的小鼠的免疫调节活性。在受到多种浓度的 AHG（0-100 μg/mL）刺激后，NK 细胞和 CTL 对 YAC-1 和 B16 细胞的表现分别优于其他细胞。此外，AHG 显著促进了 CY 诱导免疫抑制小鼠的有丝分裂原诱导脾淋巴细胞增殖。此外，AHG 以剂量依赖的方式显著增加了脾细胞 Ca 浓度和迟发型超敏反应（DTH）反应。此外，AHG 可强烈增加总抗氧化能力（T-AOC）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-PX）的活性，并降低心脏、肾脏和肝脏中的丙二醛（MDA）水平。这些发现表明 AHG 在免疫增强和保护 CY 诱导的免疫抑制和氧化损伤方面发挥了重要作用。我们的研究结果为进一步研究和可能的免疫刺激应用 AHG 提供了实验依据。
