College of Food Science and Engineering, Jinzhou Medical University, Jinzhou, 121000, Liaoning Province, China.
College of Food Science and Engineering, Jinzhou Medical College, Jinzhou, 121013, Liaoning Province, China.
Eur J Pharmacol. 2022 Jun 15;925:174999. doi: 10.1016/j.ejphar.2022.174999. Epub 2022 May 4.
Vitexin-2-O-rhamnoside (VR) is an important active substance in hawthorn, which is widely used as a food or functional food raw material; however, its immunomodulatory activities have not been extensively studied. In this study, BALB/c mice immunocompromised by cyclophosphamide (CY) were used as models to explore the effects of VR on the immunity and antioxidant capacity of mice. The results revealed that VR can restore weight to the immunosuppressed mice to varying degrees, improve spleen and thymus injury, and restore peripheral blood levels. Furthermore, it can effectively promote the proliferation of T and B lymphocytes, natural killer (NK) and cytotoxic T lymphocyte (CTL) cell activities, and the secretion and mRNA expression of cytokines IFN-γ, IL-2, IL-6, and IL-12 to 0.36, 0.34, 50.25%, 45.74%, 28.36 pg/mL or 0.68, 31.81 pg/mL or 0.74, 20.40 pg/mL or 0.75, and 19.81 pg/mL or 0.55, respectively. Moreover, it can upregulate the phosphorylation level of PI3K/Akt signaling pathway in mice immunosuppressed by CY, increase the activities of glutathione peroxidase (GSH-Px), chloramphenicol acetyltransferase (CAT), superoxide dismutase (SOD), and total antioxidant capacity (T-AOC), and decrease the level of malondialdehyde (MDA). This study provides a theoretical and experimental basis for the research and development of health products with targeted efficacy, and the development of diversified products in the hawthorn deep-processing industry.
牡荆素-2-O-鼠李糖苷（VR）是山楂中的一种重要活性物质，广泛用作食品或功能性食品原料；然而，其免疫调节活性尚未得到广泛研究。在这项研究中，使用环磷酰胺（CY）免疫抑制的 BALB/c 小鼠作为模型，探讨 VR 对小鼠免疫和抗氧化能力的影响。结果表明，VR 可以在不同程度上恢复免疫抑制小鼠的体重，改善脾和胸腺损伤，并恢复外周血水平。此外，它可以有效促进 T 和 B 淋巴细胞、自然杀伤（NK）和细胞毒性 T 淋巴细胞（CTL）的增殖以及细胞因子 IFN-γ、IL-2、IL-6 和 IL-12 的分泌和 mRNA 表达，达到 0.36、0.34、50.25%、45.74%、28.36 pg/mL 或 0.68、31.81 pg/mL 或 0.74、20.40 pg/mL 或 0.75 和 19.81 pg/mL 或 0.55。此外，它可以上调 CY 免疫抑制小鼠中 PI3K/Akt 信号通路的磷酸化水平，增加谷胱甘肽过氧化物酶（GSH-Px）、氯霉素乙酰转移酶（CAT）、超氧化物歧化酶（SOD）和总抗氧化能力（T-AOC）的活性，降低丙二醛（MDA）水平。这项研究为具有靶向功效的保健品的研究和开发以及山楂深加工产业多样化产品的开发提供了理论和实验依据。
