和 PAO1：评估精油及其主要成分萜品-4-醇的抗群体感应活性的模型。
and PAO1: Models for Evaluating Anti-Quorum Sensing Activity of Essential Oil and Its Main Component Terpinen-4-ol.
机构信息
Laboratory of Bioressources: Integrative Biology & Recovery, High Institute of Biotechnology-University of Monastir, Monastir 5000, Tunisia.
Department of Biology, College of Science, Hail, P.O. 2440, University of Ha'il, Hail 2440, Saudi Arabia.
出版信息
Molecules. 2018 Oct 17;23(10):2672. doi: 10.3390/molecules23102672.
The problem of antibiotic resistance among pathogens encourages searching for novel active molecules. The aim of the research was to assay the anti-quorum sensing (anti-QS) and antibiofilm potential of essential oil and its main constituent, terpinen-4-ol, to prevent the infections due to methicillin-resistant strains as an alternate to antibiotics. The tea tree oil (TTO) was evaluated for its potential in inhibiting QS-dependent phenomena such as violacein production in , swarming motility of PAO1, and biofilm formation in MRSA strains on glass. The results showed that terpinen-4-ol was able to inhibit MRSA strain biofilm formation on the glass strips by 73.70%. TTO inhibited the violacein production at a mean inhibitory concentration (MIC) value of 0.048 mg/mL by 69.3%. At 100 µg/mL TTO and terpinen-4-ol exhibited inhibition in swarming motility of PAO1 by 33.33% and 25%, respectively. TTO revealed anti-QS and anti-biofilm activities at very low concentrations, but it could be further investigated for new molecules useful for the treatment of MRSA infections.
病原体的抗生素耐药性问题促使人们寻找新的活性分子。本研究旨在检测茶树油及其主要成分萜品-4-醇的抗群体感应（anti-QS）和抗生物膜特性，以预防耐甲氧西林金黄色葡萄球菌（MRSA）菌株感染，作为抗生素的替代品。评估茶树油抑制依赖群体感应的现象的潜力，如在 中产生紫色素、PAO1 的群集运动和 MRSA 菌株在玻璃上的生物膜形成。结果表明，萜品-4-醇能够抑制 MRSA 菌株在玻璃条上的生物膜形成，抑制率为 73.70%。茶树油在 0.048mg/mL 的平均抑制浓度（MIC）下抑制紫色素产生，抑制率为 69.3%。在 100µg/mL 时，茶树油和萜品-4-醇对 PAO1 的群集运动的抑制率分别为 33.33%和 25%。茶树油在非常低的浓度下显示出抗群体感应和抗生物膜活性，但可以进一步研究用于治疗 MRSA 感染的新分子。
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