Center for Education and Research on Macromolecules (CERM), CESAM Research Unit, University of Liège, B-4000 Liège, Belgium; Inserm, UMR 1229, RMeS, Regenerative Medicine and Skeleton, Université de Nantes, ONIRIS, Nantes, F-44042, France.
Center for Education and Research on Macromolecules (CERM), CESAM Research Unit, University of Liège, B-4000 Liège, Belgium.
Dent Mater. 2018 Dec;34(12):1769-1782. doi: 10.1016/j.dental.2018.09.017. Epub 2018 Oct 15.
Periodontitis is an inflammatory disease that destroys the tooth-supporting attachment apparatus. Guided tissue regeneration (GTR) is a technique based on a barrier membrane designed to prevent wound space colonization by gingival cells. This study examined a new formulation composed of two polymers that could be photochemically cross-linked in situ into an interpenetrated polymer network (IPN) forming a hydrogel membrane.
We synthetized and characterized silanized hydroxypropyl methylcellulose (Si-HPMC) for its cell barrier properties and methacrylated carboxymethyl chitosan (MA-CMCS) for its degradable backbone to use in IPN. Hydrogel membranes were cross-linked using riboflavin photoinitiator and a dentistry visible light lamp. The biomaterial's physicochemical and mechanical properties were determined. Hydrogel membrane degradation was evaluated in lysozyme. Cytocompatibility was estimated by neutral red uptake. The cell barrier property was studied culturing human primary gingival fibroblasts or human gingival explants on membrane and analyzed with confocal microscopy and histological staining.
The IPN hydrogel membrane was obtained after 120s of irradiation. The IPN showed a synergistic increase in Young moduli compared with the single networks. The CMCS addition in IPN allows a progressive weight loss compared to each polymer network. Cytocompatibility was confirmed by neutral red assay. Human cell invasion was prevented by hydrogel membranes and histological sections revealed that the biomaterial exhibited a barrier effect in contact with soft gingival tissue.
We demonstrated the ability of an innovative polymer formulation to form in situ, using a dentist's lamp, an IPN hydrogel membrane, which could be an easy-to-use biomaterial for GTR therapy.
牙周炎是一种炎症性疾病，会破坏牙齿支持的附着装置。引导组织再生（GTR）是一种基于屏障膜的技术，旨在防止牙龈细胞在伤口空间中定植。本研究检查了一种由两种聚合物组成的新配方，这些聚合物可以通过光化学原位交联形成互穿聚合物网络（IPN），形成水凝胶膜。
我们合成并表征了硅烷化羟丙基甲基纤维素（Si-HPMC）的细胞屏障特性，以及用于 IPN 的可降解主链的甲基丙烯酰化羧甲基壳聚糖（MA-CMCS）。使用核黄素光引发剂和牙科可见光灯对水凝胶膜进行交联。测定了生物材料的物理化学和机械性能。在溶菌酶中评估水凝胶膜的降解。通过中性红摄取法评估细胞相容性。通过在膜上培养人原代牙龈成纤维细胞或人牙龈组织进行细胞屏障特性研究，并通过共聚焦显微镜和组织学染色进行分析。
照射 120s 后获得了 IPN 水凝胶膜。与单网络相比，IPN 表现出杨氏模量的协同增加。与每个聚合物网络相比，CMCS 的添加在 IPN 中允许逐渐的重量损失。中性红测定证实了细胞相容性。水凝胶膜可防止人细胞浸润，组织学切片显示该生物材料与柔软的牙龈组织接触时具有屏障作用。
我们证明了一种创新的聚合物配方能够在牙医灯的照射下原位形成 IPN 水凝胶膜，这可能是一种易于使用的 GTR 治疗用生物材料。
