Abdul-Monem Mohamed M, Kamoun Elbadawy A, Ahmed Dawlat M, El-Fakharany Esmail M, Al-Abbassy Fayza H, Aly Hanaa M
Dental Biomaterials Department, Faculty of Dentistry, Alexandria University, Egypt.
Polymeric Materials Research Department, Advanced Technology and New Materials Research Institute, City of Scientific Research and Technological Applications, Egypt.
J Taibah Univ Med Sci. 2021 Feb 23;16(4):529-539. doi: 10.1016/j.jtumed.2020.12.021. eCollection 2021 Aug.
Self-healing of bone from damage caused by infection, trauma, or surgical removal of cysts is limited. Generally, external intervention is needed to increase bone repair and regeneration. In this study, biocompatible light-cured hyaluronic acid hydrogels loaded with nano-hydroxyapatite and chitosan were prepared using a new photoinitiating system based on riboflavin for bone regeneration applications.
Four light-cured hydrogel groups were prepared as follows: Group I, a control group with no additions; Group II, loaded with nano-hydroxyapatite; Group III, loaded with chitosan; and Group IV, loaded with both nano-hydroxyapatite and chitosan. The new photoinitiating system consisted of riboflavin as a photoinitiator, dimethylaminoethyl methacrylate (DMAEMA) as a coinitiator (being used with riboflavin for the first time), and diphenyliodonium chloride as an accelerator. For each group, X-ray-diffraction, surface morphology by scanning electron microscope, mechanical properties, water uptake (%), and cell viability (%) were tested. The osteogenic potential was then tested in a rabbit model, and histomorphometric assessment was conducted.
In the four groups, the light-cured hydrogels were obtained after a short irradiation time of 10 s using a dental light-curing unit. The prepared hydrogels were biocompatible. Simultaneous addition of nano-hydroxyapatite and chitosan increased the mechanical properties threefold and the osteogenic potential, twofold, with a statistically significant difference compared with the control group.
Light-cured hyaluronic acid composite hydrogels loaded with nano-hydroxyapatite and chitosan-prepared by using the new photoinitiating system-are promising materials that can be used in bone regeneration applications.
因感染、创伤或手术切除囊肿导致的骨损伤自我修复能力有限。一般来说，需要外部干预来促进骨修复和再生。在本研究中，使用基于核黄素的新型光引发体系制备了负载纳米羟基磷灰石和壳聚糖的生物相容性光固化透明质酸水凝胶，用于骨再生应用。
制备四个光固化水凝胶组如下：第一组为无添加物的对照组；第二组负载纳米羟基磷灰石；第三组负载壳聚糖；第四组同时负载纳米羟基磷灰石和壳聚糖。新型光引发体系由作为光引发剂的核黄素、作为共引发剂的甲基丙烯酸二甲氨基乙酯（首次与核黄素一起使用）和作为促进剂的二苯基氯化碘鎓组成。对每组进行X射线衍射、扫描电子显微镜表面形态、力学性能、吸水率（%）和细胞活力（%）测试。然后在兔模型中测试成骨潜力，并进行组织形态计量学评估。
在四组中，使用牙科光固化装置在短至10秒的照射时间后获得了光固化水凝胶。制备的水凝胶具有生物相容性。同时添加纳米羟基磷灰石和壳聚糖使力学性能提高了三倍，成骨潜力提高了两倍，与对照组相比有统计学显著差异。
使用新型光引发体系制备的负载纳米羟基磷灰石和壳聚糖的光固化透明质酸复合水凝胶是可用于骨再生应用的有前景的材料。
