[染色质的结构组织作为决定其在照射和未照射胸腺细胞中核酸内切速率的一个因素]
[Structural organization of chromatin as a factor determining the rate of its endonucleolysis in irradiated and nonirradiated thymocytes].
作者信息
Riabchenko N I, Ivannik B P
出版信息
Radiobiologiia. 1987 Mar-Apr;27(2):155-9.
A study was made of chromatin endonucleolysis in hypotonic thymocytes incubated in digestive buffers containing different concentrations of potassium, magnesium, calcium, and mercaptoethanol. Inhibition of endonucleolysis by univalent cation during the first 20 min of incubation was followed by intensive chromatin degradation. A decrease in free potassium content retarded chromatin degradation and enhanced the inhibiting effect of the univalent cations. The regularities of changes in the rate of chromatin endonucleolysis in different digestive buffers were similar with both exposed and intact thymocytes.
对在含有不同浓度钾、镁、钙和巯基乙醇的消化缓冲液中孵育的低渗胸腺细胞中的染色质内切核酸酶解进行了研究。在孵育的前20分钟，单价阳离子对内切核酸酶解的抑制作用之后是染色质的强烈降解。游离钾含量的降低延缓了染色质降解，并增强了单价阳离子的抑制作用。在不同消化缓冲液中，暴露的和完整的胸腺细胞染色质内切核酸酶解速率的变化规律相似。
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