Konov A V, Vashchenkova I I, Ivannik B P, Riabchenko N I
Radiobiologiia. 1987 Jan-Feb;27(1):100-3.
The concentration of free thymidine (dT), excreted from thymocytes of irradiated and nonirradiated rats, was determined after incubation of cells in various digestive buffers. The release of dT from thymocytes depended upon the rate of DNA fragmentation in conditions of chromatin endonucleolysis. The increase in the thymidine content, in conditions of chromatin endonucleolysis in buffers containing no calcium ions, was only noted in thymocytes of exposed rats: this was the consequence of chromatin DNA damages already available in these cells.
在各种消化缓冲液中孵育细胞后，测定了经照射和未经照射的大鼠胸腺细胞分泌的游离胸苷（dT）浓度。胸苷从胸腺细胞中的释放取决于染色质核酸内切酶解条件下DNA片段化的速率。在不含钙离子的缓冲液中进行染色质核酸内切酶解时，仅在受照射大鼠的胸腺细胞中观察到胸苷含量增加：这是这些细胞中已存在的染色质DNA损伤的结果。
