• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

在小鼠模型中无性交的寨卡病毒性传播。

Coitus-Free Sexual Transmission of Zika Virus in a Mouse Model.

机构信息

Utah Veterinary Diagnostic Laboratory, School of Veterinary Medicine, Department of Animal, Dairy, and Veterinary Sciences, Utah State University, Logan, Utah, United States of America.

Institute for Antiviral Research, Department of Animal, Dairy, and Veterinary Sciences, Utah State University, Logan, Utah, 84322-5600, United States of America.

出版信息

Sci Rep. 2018 Oct 18;8(1):15379. doi: 10.1038/s41598-018-33528-2.

DOI:10.1038/s41598-018-33528-2
PMID:30337585
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6194026/
Abstract

Zika virus (ZIKV) is an arboviral infection that may be sexually transmitted. The present study aims to determine if accessory sex glands are a potential source of infectious virus and important in sexual transmission. Male interferon type I receptor knockout (Ifnar) mice were challenged subcutaneously with a Puerto Rican ZIKV isolate. Reproductive tissues were harvested seven days after viral challenge and artificial insemination fluid derived from epididymis or homogenized accessory sex glands (seminal plasma) was obtained. Naïve interferon type I and II receptor knockout (AG129) females were pre-treated with progesterone, and inoculated intravaginally with either epididymal flush or seminal plasma from ZIKV-infected males. ZIKV RNA was demonstrated in the artificial insemination fluid and ZIKV antigen was detected in epididymal epithelial cells but not within seminiferous tubules at the time of artificial insemination fluid collection. Peripheral viremia, demonstrated by ZIKV RNA in whole blood samples of females from each challenge group was observed. Infectious virus was present in both epididymal fluid and seminal plasma. These studies provide evidence of passage of virus from epididymal flush and seminal plasma to naïve females via artificial insemination and provides a model for the study of sexual transmission of ZIKV.

摘要

寨卡病毒（ZIKV）是一种虫媒病毒感染，可能通过性传播。本研究旨在确定附属生殖腺是否是传染性病毒的潜在来源，并在性传播中起重要作用。雄性干扰素 I 型受体敲除（Ifnar）小鼠经皮下接种波多黎各 ZIKV 分离株。在病毒接种后 7 天采集生殖组织，并获得来自附睾的人工授精液或匀浆附属生殖腺（精液）。未感染的干扰素 I 和 II 型受体敲除（AG129）雌性小鼠用孕激素预处理，然后通过阴道内接种来自 ZIKV 感染雄性的附睾冲洗液或精液进行接种。在人工授精液采集时，在人工授精液中证明了 ZIKV RNA 的存在，并且在附睾上皮细胞中检测到 ZIKV 抗原，但在曲细精管中未检测到。从每个挑战组的雌性的全血样本中检测到外周血病毒血症，通过 ZIKV RNA 证明。在附睾液和精液中均存在有感染性病毒。这些研究提供了病毒从附睾冲洗液和精液通过人工授精传递到未感染雌性的证据，并为 ZIKV 性传播的研究提供了模型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e0e/6194026/5b74144a26b6/41598_2018_33528_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e0e/6194026/da32c95b4f95/41598_2018_33528_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e0e/6194026/a00b0a7eba28/41598_2018_33528_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e0e/6194026/60d9d47feed2/41598_2018_33528_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e0e/6194026/5b74144a26b6/41598_2018_33528_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e0e/6194026/da32c95b4f95/41598_2018_33528_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e0e/6194026/a00b0a7eba28/41598_2018_33528_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e0e/6194026/60d9d47feed2/41598_2018_33528_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e0e/6194026/5b74144a26b6/41598_2018_33528_Fig4_HTML.jpg

相似文献

1
Coitus-Free Sexual Transmission of Zika Virus in a Mouse Model.在小鼠模型中无性交的寨卡病毒性传播。
Sci Rep. 2018 Oct 18;8(1):15379. doi: 10.1038/s41598-018-33528-2.
2
Zika Virus Associated Pathology and Antigen Presence in the Testicle in the Absence of Sexual Transmission During Subacute to Chronic Infection in a Mouse Model.寨卡病毒相关病理学和抗原在亚急性至慢性感染期小鼠模型中未通过性传播存在于睾丸中。
Sci Rep. 2019 Jun 6;9(1):8325. doi: 10.1038/s41598-019-44582-9.
3
Maternal Zika Virus (ZIKV) Infection following Vaginal Inoculation with ZIKV-Infected Semen in Timed-Pregnant Olive Baboons.经阴道接种感染寨卡病毒（ZIKV）精液后，定时怀孕的食蟹猴发生母源性寨卡病毒（ZIKV）感染。
J Virol. 2020 May 18;94(11). doi: 10.1128/JVI.00058-20.
4
Pathogenesis and sexual transmission of Spondweni and Zika viruses.斯庞德温尼病毒和寨卡病毒的发病机制及性传播
PLoS Negl Trop Dis. 2017 Oct 6;11(10):e0005990. doi: 10.1371/journal.pntd.0005990. eCollection 2017 Oct.
5
Zika Virus Infection, Reproductive Organ Targeting, and Semen Transmission in the Male Olive Baboon.寨卡病毒感染、雄性橄榄狒狒生殖器官靶向和精液传播。
J Virol. 2019 Dec 12;94(1). doi: 10.1128/JVI.01434-19.
6
Frequent Zika Virus Sexual Transmission and Prolonged Viral RNA Shedding in an Immunodeficient Mouse Model.免疫缺陷小鼠模型中寨卡病毒的频繁性传播及病毒RNA的长期脱落
Cell Rep. 2017 Feb 14;18(7):1751-1760. doi: 10.1016/j.celrep.2017.01.056.
7
Sexual transmission of Zika virus and other flaviviruses: A living systematic review.寨卡病毒和其他黄病毒的性传播：一项实时系统综述。
PLoS Med. 2018 Jul 24;15(7):e1002611. doi: 10.1371/journal.pmed.1002611. eCollection 2018 Jul.
8
Persistence of Zika virus RNA in the epididymis of the murine male reproductive tract.寨卡病毒 RNA 在雄性小鼠生殖道的附睾中持续存在。
Virology. 2021 Aug;560:43-53. doi: 10.1016/j.virol.2021.05.001. Epub 2021 May 14.
9
Comparative Histopathologic Lesions of the Male Reproductive Tract during Acute Infection of Zika Virus in AG129 and Ifnar Mice.AG129 和 Ifnar 小鼠急性感染寨卡病毒时雄性生殖系统的比较组织病理学病变。
Am J Pathol. 2018 Apr;188(4):904-915. doi: 10.1016/j.ajpath.2017.12.019. Epub 2018 Jan 31.
10
Infection of epididymal epithelial cells and leukocytes drives seminal shedding of Zika virus in a mouse model.附睾上皮细胞和白细胞感染可导致小鼠模型中寨卡病毒精液排出。
PLoS Negl Trop Dis. 2018 Aug 2;12(8):e0006691. doi: 10.1371/journal.pntd.0006691. eCollection 2018 Aug.

引用本文的文献

1
CD8 T cells promote ZIKV clearance and mitigate testicular damage in mice.CD8 T细胞促进小鼠体内寨卡病毒的清除并减轻睾丸损伤。
Npj Viruses. 2024 Jun 17;2(1):20. doi: 10.1038/s44298-024-00033-5.
2
Translocator protein (TSPO) is a biomarker of Zika virus (ZIKV) infection-associated neuroinflammation.转位蛋白（TSPO）是寨卡病毒（ZIKV）感染相关神经炎症的生物标志物。
Emerg Microbes Infect. 2024 Dec;13(1):2348528. doi: 10.1080/22221751.2024.2348528. Epub 2024 May 27.
3
The Susceptibility of BALB/c Mice to a Mouse-Adapted Ebola Virus Intravaginal Infection.

本文引用的文献

1
Zika Virus Shedding in Semen of Symptomatic Infected Men.寨卡病毒在有症状感染者精液中的排出。
N Engl J Med. 2018 Apr 12;378(15):1377-1385. doi: 10.1056/NEJMoa1711038.
2
Pregnancy Outcomes after ZIKV Infection in French Territories in the Americas.妊娠期寨卡病毒感染的结局：美洲法属领地的研究。
N Engl J Med. 2018 Mar 15;378(11):985-994. doi: 10.1056/NEJMoa1709481.
3
Isolation of viable Zika virus from spermatozoa.从精子中分离出活的寨卡病毒。
BALB/c 小鼠对适应鼠类的埃博拉病毒阴道感染的易感性。
Viruses. 2023 Jul 21;15(7):1590. doi: 10.3390/v15071590.
4
Establishing a Mouse Model for Sexual Transmission and Male Reproductive Tract Persistence of Ebola Virus.建立埃博拉病毒性传播和雄性生殖道持久性的小鼠模型。
J Infect Dis. 2023 Nov 15;228(Suppl 7):S554-S558. doi: 10.1093/infdis/jiad118.
5
An electron microscopic and biochemical study of the potential protective effect of ginger against Cadmium-induced testicular pathology in rats.生姜对镉诱导的大鼠睾丸病理变化潜在保护作用的电子显微镜及生化研究。
Front Physiol. 2022 Oct 3;13:996020. doi: 10.3389/fphys.2022.996020. eCollection 2022.
6
Evidence of Spreading Zika Virus Infection Caused by Males of Different Species.证据表明，不同物种的雄性可传播寨卡病毒感染。
Viruses. 2022 Sep 15;14(9):2047. doi: 10.3390/v14092047.
7
Preclinical evaluation of [F]FDG-PET as a biomarker of lymphoid tissue disease and inflammation in Zika virus infection.[F]FDG-PET 作为寨卡病毒感染中淋巴组织疾病和炎症生物标志物的临床前评估。
Eur J Nucl Med Mol Imaging. 2022 Nov;49(13):4516-4528. doi: 10.1007/s00259-022-05892-9. Epub 2022 Jul 25.
8
Zika virus persistence in the male macaque reproductive tract.寨卡病毒在雄性猕猴生殖道中的持续存在。
PLoS Negl Trop Dis. 2022 Jul 5;16(7):e0010566. doi: 10.1371/journal.pntd.0010566. eCollection 2022 Jul.
9
From Mosquito Bites to Sexual Transmission: Evaluating Mouse Models of Zika Virus Infection.从蚊子叮咬到性传播：寨卡病毒感染的小鼠模型评估。
Viruses. 2021 Nov 8;13(11):2244. doi: 10.3390/v13112244.
10
Zika Virus and Neuropathogenesis: The Unanswered Question of Which Strain Is More Prone to Causing Microcephaly and Other Neurological Defects.寨卡病毒与神经发病机制：哪种毒株更易导致小头畸形和其他神经缺陷这一未解之谜
Front Cell Neurosci. 2021 Sep 30;15:695106. doi: 10.3389/fncel.2021.695106. eCollection 2021.
Lancet Infect Dis. 2018 Feb;18(2):144. doi: 10.1016/S1473-3099(18)30020-3.
4
Comparative Histopathologic Lesions of the Male Reproductive Tract during Acute Infection of Zika Virus in AG129 and Ifnar Mice.AG129 和 Ifnar 小鼠急性感染寨卡病毒时雄性生殖系统的比较组织病理学病变。
Am J Pathol. 2018 Apr;188(4):904-915. doi: 10.1016/j.ajpath.2017.12.019. Epub 2018 Jan 31.
5
Zika virus preferentially replicates in the female reproductive tract after vaginal inoculation of rhesus macaques.在对恒河猴进行阴道接种后，寨卡病毒优先在雌性生殖道中复制。
PLoS Pathog. 2017 Jul 26;13(7):e1006537. doi: 10.1371/journal.ppat.1006537. eCollection 2017 Jul.
6
High Infection Rates for Adult Macaques after Intravaginal or Intrarectal Inoculation with Zika Virus.成年猕猴经阴道或直肠接种寨卡病毒后感染率高。
Emerg Infect Dis. 2017 Aug;23(8):1274-1281. doi: 10.3201/eid2308.170036. Epub 2017 Aug 15.
7
Baseline Prevalence of Birth Defects Associated with Congenital Zika Virus Infection - Massachusetts, North Carolina, and Atlanta, Georgia, 2013-2014.2013 - 2014年马萨诸塞州、北卡罗来纳州和佐治亚州亚特兰大市与先天性寨卡病毒感染相关的出生缺陷基线患病率
MMWR Morb Mortal Wkly Rep. 2017 Mar 3;66(8):219-222. doi: 10.15585/mmwr.mm6608a4.
8
A Mouse Model of Zika Virus Sexual Transmission and Vaginal Viral Replication.寨卡病毒性传播及阴道病毒复制的小鼠模型
Cell Rep. 2016 Dec 20;17(12):3091-3098. doi: 10.1016/j.celrep.2016.11.070.
9
Zika virus infection damages the testes in mice.寨卡病毒感染会损害小鼠的睾丸。
Nature. 2016 Dec 15;540(7633):438-442. doi: 10.1038/nature20556. Epub 2016 Oct 31.
10
Probable sexual transmission of Zika virus from a vasectomised man.寨卡病毒可能通过一名已做输精管切除术的男性发生性传播。
Lancet Infect Dis. 2016 Oct;16(10):1107. doi: 10.1016/S1473-3099(16)30320-6. Epub 2016 Sep 19.