寨卡病毒性传播及阴道病毒复制的小鼠模型
A Mouse Model of Zika Virus Sexual Transmission and Vaginal Viral Replication.
作者信息
Tang William Weihao, Young Matthew Perry, Mamidi Anila, Regla-Nava Jose Angel, Kim Kenneth, Shresta Sujan
机构信息
Division of Inflammation Biology, La Jolla Institute for Allergy & Immunology, 9420 Athena Circle, La Jolla, CA 92037, USA.
Division of Inflammation Biology, La Jolla Institute for Allergy & Immunology, 9420 Athena Circle, La Jolla, CA 92037, USA.
出版信息
Cell Rep. 2016 Dec 20;17(12):3091-3098. doi: 10.1016/j.celrep.2016.11.070.
Case reports of Zika virus (ZIKV) sexual transmission and genital persistence are mounting. Venereal transmission and genital persistence threaten public health within and beyond the range of ZIKV's mosquito vectors. In this study, we administered ZIKV into the vaginas of AG129 mice and LysMCreIFNAR C57BL/6 mice after hormonal treatments. Mice infected during estrus-like phase were resistant to vaginal infection. In contrast, when infected during diestrus-like phase, AG129 mice succumbed to infection, whereas LysMCreIFNAR mice experienced transient illness. Patency of transgenital transmission (TGT) in diestrus-like mice was demonstrated by detection of viremia and ZIKV replication in spleen and brain, and viral RNA persisted in vaginal washes as late as 10 days post-infection. In these lethal and sublethal mouse models, this study indicates that intravaginal deposition of ZIKV can cause TGT, hormonal changes in the female reproductive tract (FRT) influence transmission, and ZIKV replication persists in the FRT for several days.
寨卡病毒（ZIKV）性传播和生殖器持续感染的病例报告越来越多。性传播和生殖器持续感染对寨卡病毒蚊媒传播范围内外的公众健康都构成威胁。在本研究中，我们在激素处理后将寨卡病毒注入AG129小鼠和LysMCreIFNAR C57BL/6小鼠的阴道。在类似发情期感染的小鼠对阴道感染具有抗性。相比之下，在类似黄体期感染时，AG129小鼠会死于感染，而LysMCreIFNAR小鼠会经历短暂疾病。通过检测病毒血症以及脾脏和大脑中的寨卡病毒复制，证明了类似黄体期小鼠存在经生殖器传播（TGT），并且病毒RNA在感染后10天仍持续存在于阴道冲洗液中。在这些致死和亚致死小鼠模型中，本研究表明阴道内接种寨卡病毒可导致经生殖器传播，女性生殖道（FRT）中的激素变化会影响传播，并且寨卡病毒在女性生殖道中持续复制数天。
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