Rukhadze G G, Sergeev V A, Orliankin B G, Mogil'nyĭ Iu I
Vopr Virusol. 1987 Jan-Feb;32(1):74-80.
A swine rotavirus capable of inducing the cytopathic effect was isolated in a roller culture of Macaca rhesus kidney cells (line MA-104) after two preliminary passages in gnotobiotic piglets and colostrum-free piglets, and the isolate was designated strain K. For virus isolation, fecal specimens were treated with trypsin, and besides, trypsin was added into the maintenance medium. After 20 passages in MA-104 cell culture the swine rotavirus was adapted to pig embryo kidney cell cultures (SPEV line) in which the maximum virus accumulation, 8.0 log TCD50/ml, was achieved within 24 hours after inoculation. The virus accumulation was most marked in the presence of 10 micrograms/ml trypsin in the maintenance medium. In the roller culture, the virus multiplied to a much higher titre (approximately 100-fold) than in the stationary culture. In the course of passages the virus was shown to lose its pathogenic properties. A scheme of swine rotavirus virion structure is suggested on the basis of ultramicroscopic studies.
一株能引起细胞病变效应的猪轮状病毒，经在无菌仔猪和无初乳仔猪中初步传代两次后，在恒河猴肾细胞（MA - 104系）的转瓶培养中分离得到，该分离株被命名为K株。用于病毒分离时，粪便标本用胰蛋白酶处理，此外，维持培养基中也加入胰蛋白酶。在MA - 104细胞培养中传代20次后，该猪轮状病毒适应了猪胚胎肾细胞培养（SPEV系），接种后24小时内可在其中达到最大病毒积累量8.0 log TCD50/ml。在维持培养基中存在10微克/毫升胰蛋白酶的情况下，病毒积累最为明显。在转瓶培养中，病毒增殖到的滴度比静置培养高得多（约100倍）。在传代过程中，该病毒被证明失去了致病特性。基于超微研究提出了猪轮状病毒病毒粒子结构示意图。
