Wyatt R G, James W D, Bohl E H, Theil K W, Saif L J, Kalica A R, Greenberg H B, Kapikian A Z, Chanock R M
Science. 1980 Jan 11;207(4427):189-91. doi: 10.1126/science.6243190.
A strain of type 2 human rotavirus (Wa) was grown to relatively high titer through 14 passages in primary cultures of African green monkey kidney (AGMK) cells. This passage series was initiated with virus that had been passaged 11 times serially in newborn gnotobiotic piglets. In contrast, virus present in the stool of patient Wa as well as virus from the first, second, or third passage in piglets could not be propagated successfully in African green monkey kidney cells. Prior to each passage in cell culture, the virus was treated with trypsin and the inoculated cultures were centrifuged at low speed. Cultivation of a type 2 human rotavirus should aid attempts to characterize this virus and to develop a means of immunoprophylaxis for a serious diarrheal disease of human infants.
一株2型人类轮状病毒（Wa株）在非洲绿猴肾（AGMK）细胞原代培养物中连续传代14次后，达到了相对较高的滴度。这个传代系列起始于在新生无菌仔猪中连续传代11次的病毒。相比之下，Wa患者粪便中的病毒以及仔猪第一代、第二代或第三代传代病毒，都无法在非洲绿猴肾细胞中成功繁殖。在每次细胞培养传代之前，病毒都用胰蛋白酶处理，接种后的培养物进行低速离心。2型人类轮状病毒的培养有助于对该病毒进行特性鉴定，并开发针对人类婴儿严重腹泻疾病的免疫预防方法。
