Vanti Gulamnabi, Poondla Naresh, Manogaran Prasath, Teradal Nagappa, S Veeresh, Kaulgud Ram, Kurjogi Mahantesh
Multidisciplinary Research Unit, Karnataka Medical College and Research Institute, Hubli 580021, India.
Department of Neurology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Pharmaceuticals (Basel). 2024 Sep 18;17(9):1229. doi: 10.3390/ph17091229.
Antibiotics are successful in promoting health quality by preventing various infectious diseases and minimizing mortality and morbidity all over the world. However, the indiscriminate use of antibiotics has led to the emergence of multi-drug-resistant bacteria, which pose a serious threat to health care sector. Therefore, it is necessary to develop novel antimicrobial agents with versatile characteristics, such as antibacterial activity, low toxicity, wound healing potency, and antioxidant property. In this context, silver chitosan nanoparticles were synthesized in the present study, and their physical characterization revealed that the size of synthesized chitosan-silver nanoparticles was 14-25 nm, with positive surface charge. The functional groups and crystalline nature of the nanoparticles were confirmed by FT-IR and XRD analysis. Further, the silver chitosan nanoparticles showed antibacterial activity against two important clinical pathogens, and . The MTT assay carried out in the present study showed that the synthesized nanoparticles are non-toxic to host cells. A scratch assay on fibroblast cells (L292) demonstrated that the silver chitosan nanoparticles showed promising wound healing activity. A fluorescent DCFH-DA staining assay revealed anantioxidant property of the synthesized nanoparticles. Overall, the study emphasizes the versatile nature of synthesized chitosan-silver nanoparticles, suggesting their great compatibility for biomedical applications.
抗生素通过预防各种传染病并将全球死亡率和发病率降至最低，成功地提高了健康质量。然而，抗生素的滥用导致了多重耐药菌的出现，这对医疗保健部门构成了严重威胁。因此，有必要开发具有多种特性的新型抗菌剂，如抗菌活性、低毒性、伤口愈合能力和抗氧化性能。在此背景下，本研究合成了壳聚糖银纳米颗粒，其物理表征显示合成的壳聚糖银纳米颗粒尺寸为14 - 25纳米，表面带正电荷。通过傅里叶变换红外光谱（FT - IR）和X射线衍射（XRD）分析证实了纳米颗粒的官能团和晶体性质。此外，壳聚糖银纳米颗粒对两种重要的临床病原体显示出抗菌活性。本研究进行的MTT试验表明，合成的纳米颗粒对宿主细胞无毒。对成纤维细胞（L292）进行的划痕试验表明，壳聚糖银纳米颗粒显示出有前景的伤口愈合活性。荧光二氯荧光素二乙酸酯（DCFH - DA）染色试验揭示了合成纳米颗粒的抗氧化性能。总体而言，该研究强调了合成的壳聚糖银纳米颗粒的多功能性质，表明它们在生物医学应用中具有很好的兼容性。
