Schoenberg Markus Bo, Hao Jingcheng, Bucher Julian Nikolaus, Miksch Rainer Christoph, Anger Hubertus Johann Wolfgang, Mayer Barbara, Mayerle Julia, Neumann Jens, Guba Markus Otto, Werner Jens, Bazhin Alexandr V
Department of General, Visceral, and Transplant Surgery, Ludwig-Maximilians-University Munich, Marchioninistraße 15, 81377 Munich, Germany.
Department of Hepatobiliary Surgery, The First Affiliated Hospital of Chengdu Medical College, Chengdu 610513, China.
Cancers (Basel). 2018 Oct 18;10(10):389. doi: 10.3390/cancers10100389.
Liver resection is a curative treatment for hepatocellular carcinoma (HCC). Tumor-infiltrating leukocytes (TILs) are important players in predicting HCC recurrence. However, the invasive margin could not be confirmed as relevant for HCC. The migration of immune cells into HCC may originate from intratumoral vessels. No previous study has examined perivascular (PV) infiltration. Tumors from 60 patients were examined. Immunohistochemistry was performed against CD3, CD8, CD20, and CD66b. TILs were counted in the PV regions using an algorithm for quantification of the tumor immune stroma (QTiS). The results were correlated with overall (OS) and disease-free survival (DFS), clinical parameters, and laboratory values. PV infiltration of TILs was predominant in resected HCC. Higher PV infiltration of CD3⁺ ( = 0.016) and CD8⁺ ( = 0.028) independently predicted better OS and DFS, respectively. CD20⁺ showed a trend towards better DFS ( = 0.076). Scoring of CD3⁺, CD8⁺, and CD20⁺ independently predicted OS and DFS ( < 0.01). The amount of perivascular-infiltrating CD3⁺ cells is an independent predictor of better OS, and CD8⁺ cells independently predict prolonged DFS. Our novel perivascular infiltration scoring (PVIS) can independently predict both DFS and OS in resected HCC patients.
肝切除术是肝细胞癌（HCC）的一种治愈性治疗方法。肿瘤浸润白细胞（TILs）是预测HCC复发的重要因素。然而，浸润边缘与HCC的相关性尚未得到证实。免疫细胞向HCC的迁移可能起源于肿瘤内血管。此前尚无研究检测血管周围（PV）浸润情况。对60例患者的肿瘤进行了检查。采用免疫组织化学法检测CD3、CD8、CD20和CD66b。使用肿瘤免疫基质定量算法（QTiS）对PV区域的TILs进行计数。将结果与总生存期（OS）、无病生存期（DFS）、临床参数和实验室值进行相关性分析。TILs的PV浸润在切除的HCC中占主导地位。CD3⁺（P = 0.016）和CD8⁺（P = 0.028）的较高PV浸润分别独立预测较好的OS和DFS。CD20⁺显示出DFS较好的趋势（P = 0.076）。CD3⁺、CD8⁺和CD20⁺的评分独立预测OS和DFS（P < 0.01）。血管周围浸润的CD3⁺细胞数量是OS较好的独立预测因素，而CD8⁺细胞独立预测DFS延长。我们新的血管周围浸润评分（PVIS）可以独立预测切除的HCC患者的DFS和OS。
