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壳聚糖/银纳米复合材料用于比色检测葡萄糖分子。

Chitosan/silver nanocomposites for colorimetric detection of glucose molecules.

机构信息

School of Chemistry & Chemical Engineering, Anhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, Anhui University, Hefei, Anhui 230601, PR China; Laboratorio de Nanocelulosa y Biomateriales, Departamento de Ingeniería Química, Biotecnología y Materiales, Facultad de Ciencias Físicas y Matemáticas, Universidad de Chile, Avenida Beauchef 851, Santiago, Chile.

Department of Marine Science, Bharathidasan University, Trichy 620024, Tamil Nadu, India.

出版信息

Int J Biol Macromol. 2019 Jan;121:822-828. doi: 10.1016/j.ijbiomac.2018.10.063. Epub 2018 Oct 17.

Abstract

This study was about the simple method for the rapid colorimetric and visual detection of glucose molecules in water medium. Silver nanoparticles were spread on the chitosan surface (CS/Ag NCs) and it was characterized by UV-visible spectroscopy, fourier transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), scanning electron microscope (SEM) and transmission electron microscope (TEM). The CS/Ag NCs displayed a strong surface plasmon resonance band at 429 nm which disappears in the addition of accelerative concentrations of glucose molecules and it was attended by color alteration from yellow to colorless. The interaction of glucose molecules on the CS/Ag NCs was displayed through TEM technique. Glucose molecules was detected not only by naked eyes (from yellow to purple grey) but also by UV-vis spectrophotometer in the concentration range between 0 and 100 μM, with limit detection of 5 μM and a good linear relationship of R value = 0.99. The proposed paper was used for the application of the detection of glucose molecules in water sample with adequate outcome. This CS/Ag NCs was very simple and low cost system without using any other enzymatic catalyst and organic chromogenic agents for glucose molecules detection.

摘要

本研究提出了一种在水介质中快速比色和可视化检测葡萄糖分子的简单方法。将银纳米粒子铺展在壳聚糖表面(CS/Ag NCs),通过紫外-可见光谱、傅里叶变换红外光谱(FTIR)、X 射线衍射光谱(XRD)、扫描电子显微镜(SEM)和透射电子显微镜(TEM)对其进行了表征。CS/Ag NCs 在 429nm 处显示出很强的表面等离子体共振带,该带在加速浓度的葡萄糖分子存在下消失,并伴随着颜色从黄色变为无色的变化。通过 TEM 技术显示了葡萄糖分子在 CS/Ag NCs 上的相互作用。葡萄糖分子不仅可以通过肉眼(从黄色变为深紫色灰色)检测,还可以通过紫外-可见分光光度计在 0 到 100μM 的浓度范围内检测到,检测限为 5μM,R 值的线性关系良好,R 值为 0.99。本文提出的方法用于水样中葡萄糖分子的检测,结果令人满意。该 CS/Ag NCs 系统非常简单且成本低廉,无需使用任何其他酶催化剂和有机显色剂即可检测葡萄糖分子。

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