Zhang Feng, Ramachandran G, Mothana Ramzi A, Noman Omar M, Alobaid Waleed A, Rajivgandhi G, Manoharan N
Chest Endoscopy Minimally Invasive Area, Shandong Provincial Chest Hospital, Shandong Province 250013, China.
Department of Marine Science, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India.
Saudi J Biol Sci. 2020 Dec;27(12):3449-3455. doi: 10.1016/j.sjbs.2020.09.025. Epub 2020 Sep 18.
The development of antibiotic resistant in is an emerging thread worldwide due to the poor antimicrobial drugs. To overcome this issue, researchers are focused on plant material and their essential oils to fight against multi drug resistant bacteria. In this context, the current study was concentrated in medicinal plant of guva leaves and their essential oils to combat multi drug resistant bacterial infections. The essential oils were successfully screened and confirmed by HRLC-MS analysis. The anti-bacterial ability of the compounds were loaded into the chitosan nanoparticles and proved by FT-IR analysis. In addition, the chitosan loaded essential oils morphology was compared with chitosan alone in SEM analysis and suggested that the material was loaded successfully. Further, the anti-bacterial ability of the chitosan loaded essential oils were primarily confirmed by agar well diffusion method. At the 100 µg/mL of lowest concentration of chitosan loaded essential oils, the multi-drug resistant was inhibited with 96% and confirmed by minimum inhibition concentration experiment. Hence, all the experiments were proved that the essential oils were successfully loaded into the chitosan nanoparticles, and it has more anti-bacterial activity against multi-drug resistant
由于抗菌药物使用不当，抗生素耐药性的发展在全球范围内已成为一个新出现的问题。为了克服这一问题，研究人员将重点放在植物材料及其精油上，以对抗多重耐药细菌。在此背景下，当前的研究集中在番石榴叶这种药用植物及其精油上，以对抗多重耐药细菌感染。通过高效液相色谱 - 质谱分析成功筛选并确认了这些精油。将这些化合物的抗菌能力负载到壳聚糖纳米颗粒中，并通过傅里叶变换红外光谱分析进行了验证。此外，在扫描电子显微镜分析中，将负载精油的壳聚糖形态与单独的壳聚糖进行了比较，表明材料已成功负载。进一步地，负载精油的壳聚糖的抗菌能力主要通过琼脂孔扩散法得到证实。在负载精油的壳聚糖最低浓度为100μg/mL时，多重耐药菌受到96%的抑制，并通过最低抑菌浓度实验得到确认。因此，所有实验均证明精油已成功负载到壳聚糖纳米颗粒中，并且它对多重耐药菌具有更强的抗菌活性
