Zou Xiaofang, Cai Jingning, Li Bin, Wu Shijian
Department of Burn and Plastic Surgery, Air Force General Hospital of PLA, Beijing, China.
Medicine (Baltimore). 2019 Oct;98(43):e17482. doi: 10.1097/MD.0000000000017482.
The aim of this study was to investigate the correlation between cluster of differentiation 86 (CD86) gene rs1129055 and rs2715267 single nucleotide polymorphisms and sepsis susceptibility.One hundred twenty-five sepsis patients and 120 healthy controls were enrolled in this case-control study. CD86 polymorphisms rs1129055 and rs2715267 were genotyped through polymerase chain reaction-restriction fragment length polymorphism approach. Chi-square test was used to analyze differences in genotype and allele frequencies of the 2 polymorphisms between case and control groups. Odds ratios (ORs) and 95% confidence intervals (CIs) were used to present the association strength of the polymorphisms with sepsis susceptibility.AA genotype and A allele frequencies of CD86 rs1129055 were significantly lower in sepsis patients than in healthy controls (P < .05), revealing their significant associations with decreased disease susceptibility (OR = 0.351, 95% CI = 0.169-0.728; OR = 0.593, 95% CI = 0.415-0.847). Nevertheless, rs2715267 had no significant association with sepsis susceptibility (P > .05).AA genotype and A allele of CD86 polymorphism rs1129055 might be correlated with decreased sepsis susceptibility in Chinese Han population, but not rs2715267. Further study should be performed to verify our findings.
本研究旨在探讨分化簇86（CD86）基因rs1129055和rs2715267单核苷酸多态性与脓毒症易感性之间的相关性。本病例对照研究纳入了125例脓毒症患者和120例健康对照者。通过聚合酶链反应-限制性片段长度多态性方法对CD86多态性rs1129055和rs2715267进行基因分型。采用卡方检验分析病例组和对照组之间这两种多态性的基因型和等位基因频率差异。使用优势比（OR）和95%置信区间（CI）来表示多态性与脓毒症易感性的关联强度。脓毒症患者中CD86 rs1129055的AA基因型和A等位基因频率显著低于健康对照者（P<0.05），表明它们与疾病易感性降低显著相关（OR = 0.351，95%CI = 0.169 - 0.728；OR = 0.593，95%CI = 0.415 - 0.847）。然而，rs2715267与脓毒症易感性无显著关联（P>0.05）。CD86多态性rs1129055的AA基因型和A等位基因可能与中国汉族人群脓毒症易感性降低相关，但rs2715267并非如此。应进行进一步研究以验证我们的发现。
