Department of Textile Engineering, Yazd Branch, Islamic Azad University, Yazd, Iran.
Department of Biomedical Engineering, Yazd Branch, Islamic Azad University, Yazd, Iran.
Carbohydr Polym. 2021 Oct 1;269:118351. doi: 10.1016/j.carbpol.2021.118351. Epub 2021 Jun 17.
Here, we fabricated the platelet-rich fibrin (PRF)-loaded PCL/chitosan (PCL/CS-PRF) core-shell nanofibrous scaffold through a coaxial electrospinning method. Our goal was to evaluate the effect of CS-RPF in the core layer of the nanofibrous on the osteogenic differentiation of human mesenchymal stem cells (HMSCs). The elastic modulus of PCL/CS-PRF core-shell scaffold (44 MPa) was about 1.5-fold of PCL/CS scaffold (25 MPa). The specific surface area of the scaffolds increased from 9.98 m/g for PCL/CS scaffold to 16.66 m/g for the PCL/CS-PRF core-shell nanofibrous scaffold. Moreover, the release rate of PRF from PCL/CS-PRF nanofibrous scaffold was measured to be 24.50% after 10 days which showed slow and sustained release of PRF from the nanofibrous. The formation of Ca-P on the surface of scaffold immersed in simulated body fluid solution indicated the suitable osteoconductivity of PCL/CS-PRF core-shell nanofibrous scaffold. Also, the value of ALP activity and calcium deposited on the surface of PCL/CS-PRF core-shell nanofibrous scaffold were 81.97 U/L and 40.33 μg/scaffold, respectively after 14 days, which confirmed the significantly higher amounts of ALP and calcium deposition on the scaffold containing PRF compared to PCL/CS scaffold. Due to higher hydrophilicity and porosity of PCL/CS-PRF core-shell nanofibrous scaffold compared to PCL/CS scaffold, a better bone cell growth on surface of PCL/CS-PRF scaffold was observed. The Alizarin red-positive area was significantly higher on PCL/CS-PRF scaffold compared to PCL/CS scaffold, indicating more calcium deposition and osteogenic differentiation of HMSCs in the presence of PRF. Our findings demonstrate that PCL/CS-PRF core-shell scaffolds can provide a strong construct with improved osteogenic for bone tissue engineering applications.
在这里，我们通过同轴静电纺丝方法制造了富含血小板的纤维蛋白（PRF）负载的聚己内酯/壳聚糖（PCL/CS-PRF）核壳纳米纤维支架。我们的目标是评估核层中 CS-RPF 对人骨髓间充质干细胞（HMSCs）成骨分化的影响。PCL/CS-PRF 核壳支架的弹性模量（44MPa）约为 PCL/CS 支架的 1.5 倍（25MPa）。支架的比表面积从 PCL/CS 支架的 9.98m/g 增加到 PCL/CS-PRF 核壳纳米纤维支架的 16.66m/g。此外，PCL/CS-PRF 纳米纤维支架中 PRF 的释放率在 10 天后测量为 24.50%，表明 PRF 从纳米纤维中缓慢持续释放。浸入模拟体液溶液的支架表面上形成的 Ca-P 表明 PCL/CS-PRF 核壳纳米纤维支架具有合适的骨传导性。此外，PCL/CS-PRF 核壳纳米纤维支架表面上碱性磷酸酶（ALP）活性和钙沉积的数值分别为 14 天后的 81.97U/L 和 40.33μg/支架，证实了含有 PRF 的支架上 ALP 和钙沉积的量明显高于 PCL/CS 支架。与 PCL/CS 支架相比，PCL/CS-PRF 核壳纳米纤维支架具有更高的亲水性和孔隙率，因此观察到表面上更好的骨细胞生长。与 PCL/CS 支架相比，PCL/CS-PRF 支架上茜素红阳性面积明显更高，表明在 PRF 的存在下 HMSCs 的钙沉积和成骨分化更多。我们的研究结果表明，PCL/CS-PRF 核壳支架可为骨组织工程应用提供具有增强成骨作用的强结构。
