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对HeLa细胞核因子I识别位点的接触点分析揭示了在DNA螺旋一侧的对称结合。

Contactpoint analysis of the HeLa nuclear factor I recognition site reveals symmetrical binding at one side of the DNA helix.

作者信息

de Vries E, van Driel W, van den Heuvel S J, van der Vliet P C

出版信息

EMBO J. 1987 Jan;6(1):161-8. doi: 10.1002/j.1460-2075.1987.tb04734.x.

DOI:10.1002/j.1460-2075.1987.tb04734.x
PMID:3034574
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC553372/
Abstract

Nuclear factor I (NFI) is a HeLa sequence-specific DNA-binding protein that is required for initiation of adenovirus (Ad) DNA replication and may be involved in the expression of several cellular genes. The interaction between NFI and its binding site on the Ad2 origin has been studied. Methylation interference and protection, u.v. irradiation of 5-BrdU substituted DNA and ethylation interference revealed major groove contacts with G and T, and phosphate backbone contacts. Computer stereographics show that the contacts are located in two blocks showing dyad symmetry to each other and 22 out of 23 contacts are accessible from one side of the helix. Inversion of the NFI binding site did not change the NFI dependent stimulation of Ad2 DNA replication in a reconstituted system. All data are compatible with NFI binding as a dimer at one side of the DNA helix.

摘要

核因子I（NFI）是一种HeLa序列特异性DNA结合蛋白，腺病毒（Ad）DNA复制起始需要该蛋白，并且它可能参与多个细胞基因的表达。已经研究了NFI与其在Ad2起始位点上的结合位点之间的相互作用。甲基化干扰和保护、5-溴脱氧尿苷（5-BrdU）取代DNA的紫外线照射以及乙基化干扰揭示了与鸟嘌呤（G）和胸腺嘧啶（T）的大沟接触以及磷酸主链接触。计算机立体图显示，这些接触位于两个相互呈二元对称的区域，并且23个接触点中有22个可从螺旋的一侧接近。在重组系统中，NFI结合位点的反转并未改变NFI对Ad2 DNA复制的依赖性刺激。所有数据都与NFI作为二聚体在DNA螺旋一侧的结合相一致。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1614/553372/08d175e4b511/emboj00241-0165-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1614/553372/1dfa9cabee85/emboj00241-0160-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1614/553372/01ffcb4c0261/emboj00241-0161-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1614/553372/3130d843ef63/emboj00241-0161-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1614/553372/adf5745dd561/emboj00241-0162-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1614/553372/1c9ed95e3429/emboj00241-0162-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1614/553372/bb782539b466/emboj00241-0163-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1614/553372/08d175e4b511/emboj00241-0165-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1614/553372/1dfa9cabee85/emboj00241-0160-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1614/553372/01ffcb4c0261/emboj00241-0161-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1614/553372/3130d843ef63/emboj00241-0161-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1614/553372/adf5745dd561/emboj00241-0162-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1614/553372/1c9ed95e3429/emboj00241-0162-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1614/553372/bb782539b466/emboj00241-0163-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1614/553372/08d175e4b511/emboj00241-0165-a.jpg

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