Müller U, Stephan D, Philippsen P, Steinmetz M
EMBO J. 1987 Feb;6(2):369-73. doi: 10.1002/j.1460-2075.1987.tb04764.x.
Over the past few years six gene clusters have been isolated from the major histocompatibility complex (MHC) of the BALB/c mouse encompassing a total of 1600 kb of DNA and 48 genes. The molecular distances between these gene clusters and the orientation of four of the six clusters on chromosome 17 is not known. Here we use pulse-field gradient gels and Southern blot hybridization to establish large-scale genomic restriction maps covering several hundreds of kb surrounding the three gene clusters located in the K, I, S, and D regions of the MHC. Comparison of the maps orients the complement gene clusters in the S region with the 21-OHB gene pointing towards the K end and the C2 gene pointing towards the D end of the MHC. The distances between the E alpha and 21-OHB genes is 430 kb and between the C2 and TNF-alpha genes at least 420 kb.
在过去几年里，已从BALB/c小鼠的主要组织相容性复合体（MHC）中分离出六个基因簇，这些基因簇总共包含1600 kb的DNA和48个基因。这六个基因簇之间的分子距离以及其中四个基因簇在17号染色体上的方向尚不清楚。在此，我们使用脉冲场梯度凝胶和Southern印迹杂交技术，构建了覆盖MHC的K、I、S和D区域中三个基因簇周围数百kb的大规模基因组限制性图谱。通过图谱比较，确定了S区域中补体基因簇的方向，其中21-OHB基因指向MHC的K端，C2基因指向MHC的D端。Eα基因与21-OHB基因之间的距离为430 kb，C2基因与TNF-α基因之间的距离至少为420 kb。
