Division of Environmental Health Sciences, College of Public Health, The Ohio State University, Columbus, OH 43210, USA.
Human Nutrition Program, Department of Human Sciences, College of Education and Human Ecology, The Ohio State University, Columbus, OH 43210, USA.
Int J Environ Res Public Health. 2018 Oct 19;15(10):2293. doi: 10.3390/ijerph15102293.
Airborne particulate matter (PM) exposure remains the leading environmental risk factor for disease globally. Interventions to mitigate the adverse effects of PM are required, since there is no discernible threshold for its effects, and exposure reduction approaches are limited. The mitigation of PM (specifically diesel exhaust particles (DEP))-induced release of pro-inflammatory cytokines interleukin-6 (IL-6) and interleukin-8 (IL-8) and vasoconstrictor endothelin-1 (ET-1) after 24 and 48 h of exposure by pre-treatment with individual pure, combined pure, and an oil formulation of two fish oil omega-3 polyunsaturated fatty acids (ω-3 PUFAs), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA) were all tested at an equivalent concentration of 100 µM in vitro in human umbilical vein endothelial cells. The PUFAs and fish oil formulation completely mitigated or diminished the DEP-induced release of IL-6, IL-8, and ET-1 by 14⁻78%. DHA was more effective in reducing the levels of the DEP-induced release of the cytokines, especially IL-6 after 48 h of DEP exposure in comparison to EPA ( < 0.05), whereas EPA seemed to be more potent in reducing ET-1 levels. The potential of fish ω-3 PUFAs to mitigate PM-induced inflammation and vasoactivity was demonstrated by this study.
空气中的颗粒物（PM）暴露仍然是全球疾病的主要环境风险因素。需要采取干预措施来减轻 PM 的不良影响，因为其影响没有明显的阈值，而且暴露减少的方法有限。本研究旨在检测两种鱼油 ω-3 多不饱和脂肪酸（ω-3PUFAs）、二十二碳六烯酸（DHA）和二十碳五烯酸（EPA）的纯品、组合纯品和油配方在体外人脐静脉内皮细胞中以 100µM 等效浓度预处理后，24 小时和 48 小时时分别对 PM（特别是柴油废气颗粒（DEP））诱导的促炎细胞因子白细胞介素 6（IL-6）和白细胞介素 8（IL-8）以及血管收缩剂内皮素-1（ET-1）的释放的缓解作用。PUFAs 和鱼油配方完全缓解或减少了 14%至 78%的 DEP 诱导的 IL-6、IL-8 和 ET-1 的释放。与 EPA 相比，DHA 在减少细胞因子尤其是 DEP 暴露 48 小时后 IL-6 的释放水平方面更为有效（<0.05），而 EPA 似乎在降低 ET-1 水平方面更为有效。本研究证明了鱼 ω-3 PUFAs 减轻 PM 诱导的炎症和血管活性的潜力。
