Dept. of Biochemistry and Molecular Biology, University of Cordoba, Agrifood Campus of International Excellence (ceiA3), 14071 Cordoba, Spain; Plant Molecular Biology Dept., Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. Bl 21, 1113 Sofia, Bulgaria.
Dept. of Biochemistry and Molecular Biology, University of Cordoba, Agrifood Campus of International Excellence (ceiA3), 14071 Cordoba, Spain.
Plant Sci. 2018 Nov;276:1-13. doi: 10.1016/j.plantsci.2018.07.007. Epub 2018 Jul 21.
Quercus ilex is a dominant tree species in the Mediterranean region with double economic and ecological importance and increasing use in reforestation. Seedling establishment is extremely vulnerable to environmental stresses, particularly drought. A time course study on physiological and proteomic response of holm oak to water limitation stress and recovery during early heterotrophic growth is reported. Applied stress led to diminution in plant water content and root growth, oxidative stress in roots and some alterations in the anti-oxidative protection. Plant parts differed substantially in soluble sugar and free phenolic content, and in their changes during stress and recovery. Proteomic response in holm oak roots and cotyledons was estimated using combined 1-DE/2-DE approach and protein identification by MALDI TOF-TOF PMF and MS/MS. A total of 127 differentially abundant protein species (DAPs) were identified. DAPs related to starch metabolism, lipid to sugar conversion, reserve proteins and their mobilization were typical for cotyledons. DAPs in roots were involved in sugar utilization, secondary metabolism and defense, including pathogenesis related proteins from PR-5 and PR-10 families. Results emphasize specific proteome signatures of separate plant parts as well as importance of sink-source interaction between root and cotyledon in the time course of stress and in recovery.
欧洲栓皮栎是地中海地区的优势树种，具有双重经济和生态重要性，并且在重新造林中的应用越来越多。幼苗的建立对环境胁迫非常敏感，特别是干旱。本文报道了欧洲栓皮栎幼苗对水分限制胁迫的生理和蛋白质组学响应及其在早期异养生长期间恢复的时程研究。施加的胁迫导致植物含水量和根生长减少，根中产生氧化应激，并对抗氧化保护系统产生一些改变。在可溶性糖和游离酚含量及其在胁迫和恢复过程中的变化方面，植物各部分有很大差异。使用联合 1-DE/2-DE 方法和 MALDI TOF-TOF PMF 和 MS/MS 进行鉴定，对欧洲栓皮栎根和子叶的蛋白质组学响应进行了估计。共鉴定出 127 种差异丰度蛋白（DAP）。与淀粉代谢、脂质到糖的转化、储备蛋白及其动员相关的 DAP 是子叶的典型特征。根中的 DAP 涉及糖利用、次生代谢和防御，包括来自 PR-5 和 PR-10 家族的病程相关蛋白。研究结果强调了植物各部分的特定蛋白质组特征，以及根和子叶之间在胁迫和恢复过程中的源库相互作用的重要性。
