School of Criminal Justice, University of Lausanne, Switzerland.
Section of Chemistry and Chemical Engineering, Ecole Polytechnique Fédérale de Lausanne, Switzerland.
Talanta. 2019 Jan 15;192:135-141. doi: 10.1016/j.talanta.2018.09.012. Epub 2018 Sep 6.
Nowadays, Gas Chromatography Mass Spectrometry (GC-MS) is mainly used in forensic sciences but suffers from limitations when the analysed compounds are thermally instable as it is the case for THC-A (Tetrahydrocannabinolic Acid) which is converted into Δ-THC (Δ-Tetrahydrocannabinol) that subsequently partially degrades. We propose herein a Fast High Pressure Liquid Chromatography (Fast-HPLC-DAD) method which allows the efficient separation of CBN (Cannabinol), CBD (Cannabidiol), THC-A and Δ-THC, the major cannabinoids compounds found in cannabis plants in less than 5 min. Our method allows also the proper quantification of Δ-THC in plant extracts using an external calibration method with a very good accuracy as pointed out by a recovery of 100.53 ± 3.12%. It is also an interesting low cost alternative to Ultra High Pressure Liquid Chromatography (UPLC) for routine analyses in forensic sciences.
如今，气相色谱-质谱联用（GC-MS）主要用于法医学，但当分析的化合物热不稳定时，它会受到限制，就像四氢大麻酚酸（THC-A）一样，它会转化为Δ-THC（Δ-四氢大麻酚），随后会部分降解。我们在此提出了一种快速高压液相色谱（Fast-HPLC-DAD）方法，该方法能够在不到 5 分钟的时间内有效分离大麻植物中发现的主要大麻素化合物，包括大麻酚（CBN）、大麻二酚（CBD）、THC-A 和 Δ-THC。我们的方法还允许使用外部校准方法对植物提取物中的 Δ-THC 进行适当的定量，其准确度非常高，回收率为 100.53±3.12%。对于法医学中的常规分析，它也是一种比超高效液相色谱（UPLC）更具成本效益的选择。
