Nakos Ioannis, Kadoglou Nikolaos P E, Gkeka Paraskevi, Tzallas Alexandros T, Giannakeas Nikolaos, Tsalikakis Dimitrios G, Katsimpoulas Michalis, Mantziaras Georgios, Kostomitsopoulos Nikolaos, Liapis Christos D, Kakisis John
Biomedical Research Foundation, Center of Clinical, Experimental Surgery, and Translational Research, Academy of Athens, Athens, Greece
Biomedical Research Foundation, Center of Clinical, Experimental Surgery, and Translational Research, Academy of Athens, Athens, Greece.
In Vivo. 2018 Nov-Dec;32(6):1433-1441. doi: 10.21873/invivo.11396.
BACKGROUND/AIM: Exercise training usually complements pharmacological therapy of type 1 diabetes mellitus, however, little is known about its impact on cardiac autonomic neuropathy. Our aim was to evaluate the impact of exercise on electrocardiographic parameters and heart rate variability in diabetic rats.
Wistar rats were randomly assigned to four groups (n=12): Sedentary control (SC), sedentary diabetic (SD), exercise control (EC), and exercise diabetic (ED). Diabetes was induced by a single intraperitoneal injection of streptozotocin (45 mg/kg). Exercise groups underwent 8 weeks of training on a treadmill. At the end of the study, echocardiography was performed and continuous electrocardiographic recording was obtained by intra-abdominally implanted telemetric devices. Diabetes induction significantly reduced the heart rate and increased the blood glucose level (p<0.001) and R-wave amplitude (p<0.05). Frequency-domain spectral variables were also analyzed. The SD group had a significantly lower absolute high-frequency component (p<0.05) and higher normalized low-frequency component, as well as low-frequency power divided by the high-frequency power ratio when compared to the SC and EC groups (p<0.05). All these diabetes-related adverse changes in heart rate variability parameters were significantly reversed by exercise training (p<0.05). Overall, our study shows that early initiation of systemic exercise training prevents the development of cardiac autonomic neuropathy in rats with type 1 diabetes mellitus, by favorable change in the balance between parasympathetic and sympathetic activity.
背景/目的：运动训练通常是1型糖尿病药物治疗的辅助手段，然而，其对心脏自主神经病变的影响却鲜为人知。我们的目的是评估运动对糖尿病大鼠心电图参数和心率变异性的影响。
将Wistar大鼠随机分为四组（n = 12）：久坐对照组（SC）、久坐糖尿病组（SD）、运动对照组（EC）和运动糖尿病组（ED）。通过单次腹腔注射链脲佐菌素（45 mg/kg）诱导糖尿病。运动组在跑步机上进行8周训练。在研究结束时，进行超声心动图检查，并通过腹腔内植入的遥测装置进行连续心电图记录。糖尿病诱导显著降低了心率，升高了血糖水平（p < 0.001）和R波振幅（p < 0.05）。还分析了频域频谱变量。与SC组和EC组相比，SD组的绝对高频成分显著降低（p < 0.05），归一化低频成分更高，以及低频功率除以高频功率比值更高（p < 0.05）。运动训练显著逆转了所有这些与糖尿病相关的心率变异性参数的不良变化（p < 0.05）。总体而言，我们的研究表明，早期开始全身运动训练可通过改善副交感神经和交感神经活动之间的平衡，预防1型糖尿病大鼠心脏自主神经病变的发展。
