Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Sci Rep. 2018 Oct 22;8(1):15593. doi: 10.1038/s41598-018-32756-w.
Carbon dots and FeO@Au were synthesized to develop a new biosensor to detect DNA target. We investigated the photoluminescence property of carbon dots (CDs) in the presence of FeO-capped Au (FeO@Au). Firstly, we designed two dedicated probes for unique long sequence region of human T-lymphotropic virus type 1 genome. One of the probes was covalently bound to the CDs. In the absence of target, CDs-probe was adsorbed on the surface of FeO@Au through two possible mechanisms, leading to quenching the fluorescence emission of CDs. The fluorescence emission of CDs was recovered in the presence of target since double-stranded DNA cannot adsorb on the FeO@Au. Also, FeO@Au can adsorb the unhybridized oligonucleotides and improves the accuracy of detection. The specificity of the proposed biosensor was confirmed by BLAST search and assessed by exposing the biosensor to other virus targets. The experimental detection limit of the biosensor was below 10 nM with linear range from 10 to 320 nM.
合成了碳点和 FeO@Au，以开发一种新的用于检测 DNA 靶标的生物传感器。我们研究了碳点 (CDs) 在 FeO 覆盖的 Au (FeO@Au) 存在下的光致发光特性。首先，我们设计了两个专用探针用于人类 T 淋巴细胞病毒 1 基因组的独特长序列区域。其中一个探针与 CDs 共价结合。在没有靶标的情况下，CDs 探针通过两种可能的机制吸附在 FeO@Au 表面，导致 CDs 的荧光发射猝灭。由于双链 DNA 不能吸附在 FeO@Au 上，因此在存在靶标的情况下，CDs 的荧光发射得到恢复。此外，FeO@Au 可以吸附未杂交的寡核苷酸，提高了检测的准确性。通过 BLAST 搜索证实了该生物传感器的特异性，并通过将生物传感器暴露于其他病毒靶标来评估其特异性。该生物传感器的实验检测限低于 10 nM，线性范围为 10 至 320 nM。
