Kim Mina, Nam Dong-Geon, Kim Sang-Bum, Im Pureum, Choe Jeong-Sook, Choi Ae-Jin
Division of Functional Food & Nutrition Department of Agrofood Resources National Institute of Agricultural Science Rural Development Administration Jeonju Korea.
Food Sci Nutr. 2018 Aug 22;6(7):1904-1913. doi: 10.1002/fsn3.762. eCollection 2018 Oct.
Dietary supplementation with lactic acid bacteria to maintain or improve intestinal health is advocated. spp. are present in different fermented vegetable-based foods like , as well as in the normal gastrointestinal (GI) tract of humans. strains have been proposed as potential probiotics. Freeze-drying is a promising treatment method for these strains for industrial applications and to increase the accessibility of their health-promoting benefits. Moreover, probiotic strains need to be able to survive in the host GI tract, and acid and bile are both environmental stressors that can reduce strain survival. Therefore, this study evaluated the effect of the combination of protective agents on the acid and bile resistance of JW15 after freeze-drying. A protective agent combination with a 1:1 ratio of 5 g + 5 g/100 ml w/v soy flour + yeast extract (SFY) retained nearly 100% viability after freeze-drying and was resistant to artificial bile acids. Remarkably, skim milk + soy flour (SSF) was resistant to an acidic solution, and the viability of JW15 in artificial gastric acid was enhanced when treated with this mixture. Furthermore, SFY and SSF were found to maintain high numbers of viable cells with a low specific rate of cell death () after storage at 50°C, 60°C, and 70°C. These results support an effective probiotic formulation system with a high number of viable cells, and its protective effects can be leveraged in the development of probiotic products with health benefits.
提倡通过补充乳酸菌来维持或改善肠道健康。乳酸菌存在于不同的以发酵蔬菜为基础的食物中，如泡菜，也存在于人类正常的胃肠道中。某些乳酸菌菌株已被提议作为潜在的益生菌。冷冻干燥是一种很有前景的处理方法，可用于这些菌株的工业应用，并提高其促进健康益处的可及性。此外，益生菌菌株需要能够在宿主胃肠道中存活，而酸和胆汁都是可能降低菌株存活率的环境应激源。因此，本研究评估了保护剂组合对冻干后JW15乳酸菌耐酸和耐胆汁能力的影响。一种由5 g + 5 g/100 ml重量/体积比的大豆粉 + 酵母提取物（SFY）按1:1比例组成的保护剂组合在冻干后保留了近100%的活力，并且对人工胆汁酸具有抗性。值得注意的是，脱脂牛奶 + 大豆粉（SSF）对酸性溶液具有抗性，并且用这种混合物处理后，JW15乳酸菌在人工胃酸中的活力增强。此外，发现SFY和SSF在50°C、60°C和70°C储存后能保持大量的活细胞，且细胞死亡的比速率较低。这些结果支持了一种具有大量活细胞的有效益生菌制剂系统，其保护作用可用于开发具有健康益处的益生菌产品。
