Department of Plastic Surgery, Shengjing Hospital, Affiliated Hospital of China Medical University, Shenyang, Liaoning, 110004, China.
Department of Tissue Engineering, School of Fundamental Science, China Medical University, Shenyang, Liaoning, 110122, China.
J Biomed Mater Res B Appl Biomater. 2019 Jul;107(5):1684-1694. doi: 10.1002/jbm.b.34261. Epub 2018 Oct 23.
Adipose tissue engineering is considered as a promising treatment for repairing soft tissue defects. The decellularized extracellular matrix (ECM) is becoming the research focus in tissue engineering for its tissue specificity. In this study, the human adipose tissue liposucted from healthy people were decellularized by a series of mechanical, chemical, and enzymatic methods. The components of cell and lipid were effectively removed, whereas the collagens and other ingredients in adipose tissue were retained in the human decellularized adipose tissue (hDAT). Then the extracted hDAT was further fabricated into injectable hydrogel, which could be self-assembled to form gel under certain condition. The hDAT hydrogel was nontoxic to human adipose-derived stem cells (ADSCs) and could spontaneously induce adipogenic differentiation in vitro. It was highly biocompatible and could not cause inflammation and rejection after being implanted subcutaneously. The hDAT hydrogel developed in this study will be one of the available choices for soft tissue enlargement and cosmetic fillers because of its noninvasive in collection and implantation process. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1684-1694, 2019.
脂肪组织工程被认为是修复软组织缺损的一种有前途的治疗方法。去细胞细胞外基质 (ECM) 因其组织特异性而成为组织工程研究的焦点。在这项研究中，从健康人身上吸脂的脂肪组织通过一系列机械、化学和酶方法进行去细胞化处理。细胞和脂质成分被有效去除，而脂肪组织中的胶原蛋白和其他成分则保留在人去细胞脂肪组织 (hDAT) 中。然后将提取的 hDAT 进一步制成可注射水凝胶，在一定条件下可自行组装形成凝胶。hDAT 水凝胶对人脂肪源性干细胞 (ADSCs) 无毒，可在体外自发诱导成脂分化。它具有高度的生物相容性，在皮下植入后不会引起炎症和排斥反应。由于采集和植入过程无创伤，本研究中开发的 hDAT 水凝胶将成为软组织增大和美容填充物的可选材料之一。© 2018 Wiley Periodicals, Inc. J 生物材料 Res 部分 B: 应用生物材料 107B: 1684-1694, 2019.
