Institute of Insect Science, Hunan Agriculture University, Changsha 410128, China; Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Institute of Plant and Environmental Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
Institute of Plant and Environmental Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
Int J Biol Macromol. 2019 Jan;121:1135-1144. doi: 10.1016/j.ijbiomac.2018.10.143. Epub 2018 Oct 21.
The cotton mealybug, Phenacoccus solenopsis Tinsley (Hemipeta: Pseudoccoccidae), is an aggressively invasive pest causing huge economic losses of crops around the world. In this study, we developed genome-wide microsatellites for population genetic analysis of P. solenopsis. We obtained a random genome of P. solenopsis with a size of 267.07 Mb and scaffold N50 of 14.12 Kb. In total 115,639 microsatellites were isolated from the genome, of which those with trinucleotide motifs were the most abundant. Forty-two polymorphic loci were selected for primer validation based on three populations. Allele numbers varied from 2 to 5 with an average value of 2.5 per locus, and allelic richness ranged from 1.00 to 4.48. The observed heterozygosity (H) and expected heterozygosity (H) ranged from 0.00 to 0.92 and 0.00 to 0.73, respectively. Population genetic structure analysis based on the developed markers revealed strong differentiation between three populations of P. solenopsis collected from its invasive range in China. The microsatellites developed in our study should provide efficient genetic markers for population level studies of P. solenopsis to reveal invasion history and patterns of dispersal.
棉红铃虫（Phenacoccus solenopsis Tinsley）（半翅目：假盾蚧科）是一种具有侵略性的入侵害虫，对世界各地的农作物造成了巨大的经济损失。在本研究中，我们开发了用于棉红铃虫种群遗传分析的基因组广泛微卫星。我们获得了大小为 267.07 Mb 的棉红铃虫随机基因组和支架 N50 为 14.12 Kb。总共从基因组中分离出 115639 个微卫星，其中三核苷酸基序的微卫星最多。根据三个种群，选择了 42 个多态性位点进行引物验证。等位基因数从 2 到 5 不等，平均每个位点为 2.5，等位基因丰富度从 1.00 到 4.48 不等。观察到的杂合度（H）和预期杂合度（H）从 0.00 到 0.92 和 0.00 到 0.73 不等。基于开发的标记进行的种群遗传结构分析表明，从中国入侵范围采集的棉红铃虫的三个种群之间存在强烈分化。本研究开发的微卫星应为棉红铃虫种群水平研究提供有效的遗传标记，以揭示其入侵历史和扩散模式。
