Faculty of Pharmaceutical Sciences, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand.
Faculty of Pharmaceutical Sciences, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand; Nano-Cosmeceuticals Laboratory, National Nanotechnology Center, National Science and Technology Development Agency, Klong Luang, Pathumthani 12120, Thailand.
J Integr Med. 2018 Nov;16(6):427-434. doi: 10.1016/j.joim.2018.10.002. Epub 2018 Oct 5.
The leaves of Chromolaena odorata, a highly invasive shrub found growing wild worldwide, are traditionally used for wound healing. Due to its high flavonoid contents, we aimed to find a new application for this plant. Preliminary tests using its ethanolic leaf extract showed that it could suppress the accumulation of lipids in adipocytes. We therefore studied the anti-adipogenic effect of several C. odorata leaf extracts and the relationship between molecular structure and bio-activity of its isolated flavonoid constituents using 3T3-L1 preadipocytes/adipocytes as a model.
Three leaf extracts and thirteen flavonoids isolated from C. odorata were tested for their effect on lipid accumulation in 3T3-L1 adipocytes using AdipoRed reagent, with quercetin as the positive control. The effects of active flavonoids on the adipocytes were confirmed by oil red O staining and visualized under a light microscope.
n-Hexane and ethyl acetate extracts of C. odorata leaves displayed anti-adipogenic activity. The latter extract was the more potent one, especially at 40 µg/mL. Four flavonoids, pectolinarigenin, kaempferide, 4,2'-dihydroxy-4',5',6'-trimethoxychalcone and dillenetin, exhibited significant, concentration-dependent inhibitory effects on lipid accumulation in 3T3-L1 adipocytes. The most potent flavonoid obtained in this study was 4,2'-dihydroxy-4',5',6'-trimethoxychalcone, which caused 75% and 90% inhibition of cellular lipid accumulation at 30 and 50 µmol/L, respectively. Both kaempferide and 4,2'-dihydroxy-4',5',6'-trimethoxychalcone were major constituents in the ethyl acetate extract of this plant.
C. odorata leaves contained several flavonoids with anti-adipogenic effects against lipid accumulation in 3T3-L1 adipocytes. The plant, normally considered a useless weed, may actually provide an abundant source of biologically active flavonoids.
变色臭牡丹是一种广泛分布于世界各地的高度入侵性灌木，其叶子被传统上用于伤口愈合。由于其含有丰富的类黄酮，我们旨在为这种植物寻找新的应用。使用其乙醇叶提取物进行的初步测试表明，它可以抑制脂肪细胞中脂质的积累。因此，我们使用 3T3-L1 前体脂肪细胞/脂肪细胞作为模型，研究了几种变色臭牡丹叶提取物的抗脂肪生成作用以及其分离的类黄酮成分的分子结构与生物活性之间的关系。
使用 AdipoRed 试剂测试了三种叶提取物和从变色臭牡丹中分离出的 13 种类黄酮对 3T3-L1 脂肪细胞中脂质积累的影响，以槲皮素作为阳性对照。通过油红 O 染色和在光显微镜下观察，证实活性类黄酮对脂肪细胞的影响。
变色臭牡丹叶的正己烷和乙酸乙酯提取物显示出抗脂肪生成活性。后者提取物的作用更强，尤其是在 40μg/ml 时。四种类黄酮，即 pectolinarigenin、山柰酚、4,2'-二羟基-4',5',6'-三甲氧基查耳酮和二氢杨梅素，对 3T3-L1 脂肪细胞中脂质积累表现出显著的、浓度依赖性的抑制作用。在本研究中获得的最有效类黄酮是 4,2'-二羟基-4',5',6'-三甲氧基查耳酮，其在 30 和 50μmol/L 时分别导致细胞内脂质积累抑制 75%和 90%。山柰酚和 4,2'-二羟基-4',5',6'-三甲氧基查耳酮均为该植物乙酸乙酯提取物的主要成分。
变色臭牡丹叶含有几种具有抗脂肪生成作用的类黄酮，可以抑制 3T3-L1 脂肪细胞中脂质的积累。这种通常被认为是无用杂草的植物实际上可能是生物活性类黄酮的丰富来源。
