School of Pharmacy, International Medical University, Bukit Jalil, 57000 Kuala Lumpur, Malaysia; Department of Human Biology, International Medical University, Bukit Jalil, 57000 Kuala Lumpur, Malaysia.
J Zhejiang Univ Sci B. 2014 Mar;15(3):295-302. doi: 10.1631/jzus.B1300123.
This study examined the anti-adipogenic effects of extracts of Ficus deltoidea var. deltoidia and var. angustifolia, a natural slimming aid, on 3T3-L1 adipocytes.
Methanol and water extracts of leaves of the F. deltoidea varieties were analyzed to determine their total flavonoid content (TFC) and total phenolic content (TPC), respectively. The study was initiated by determining the maximum non-toxic dose (MNTD) of the methanol and water extracts for 3T3-L1 preadipocytes. Possible anti-adipogenic effects were then examined by treating 2-d post confluent 3T3-L1 preadipocytes with either methanol extract or water extract at MNTD and half MNTD (½MNTD), after which the preadipocytces were induced to form mature adipocytes. Visualisation and quantification of lipid content in mature adipocytes were carried out through oil red O staining and measurement of optical density (OD) at 520 nm, respectively.
The TFCs of the methanol extracts were 1.36 and 1.97 g quercetin equivalents (QE)/100 g dry weight (DW), while the TPCs of the water extracts were 5.61 and 2.73 g gallic acid equivalents (GAE)/100 g DW for var. deltoidea and var. angustilofia, respectively. The MNTDs determined for methanol and water extracts were (300.0 ± 28.3) and (225.0 ± 21.2) µg/ml, respectively, for var. deltoidea, while much lower MNTDs [(60.0 ± 2.0) µg/ml for methanol extracts and (8.0 ± 1.0) µg/ml for water extracts] were recorded for var. angustifolia. Studies revealed that the methanol extracts of both varieties and the water extracts of var. angustifolia at either MNTD or ½MNTD significantly inhibited the maturation of preadipocytes.
The inhibition of the formation of mature adipocytes indicated that leaf extracts of F. deltoidea could have potential anti-obesity effects.
本研究考察了天然瘦身辅助剂三角枫和窄叶三角枫叶提取物对 3T3-L1 脂肪细胞的抗脂肪生成作用。
分析三角枫品种叶片的甲醇和水提取物，分别测定其总黄酮含量（TFC）和总酚含量（TPC）。首先确定 3T3-L1 前脂肪细胞甲醇和水提取物的最大无毒剂量（MNTD）。然后，用 MNTD 和半 MNTD（½MNTD）处理 2 天融合后的 3T3-L1 前脂肪细胞，以研究可能的抗脂肪生成作用，然后诱导前脂肪细胞形成成熟脂肪细胞。通过油红 O 染色和在 520nm 处测量吸光度（OD）分别对成熟脂肪细胞中的脂质含量进行可视化和定量。
甲醇提取物的 TFC 分别为 1.36 和 1.97g 槲皮素当量（QE）/100g 干重（DW），而水提取物的 TPC 分别为 5.61 和 2.73g 没食子酸当量（GAE）/100g DW 用于三角枫和窄叶三角枫，分别。甲醇和水提取物的 MNTD 分别为（300.0±28.3）和（225.0±21.2）µg/ml，用于三角枫品种，而窄叶三角枫的 MNTD 低得多[甲醇提取物为（60.0±2.0）µg/ml，水提取物为（8.0±1.0）µg/ml]。研究表明，两种品种的甲醇提取物和水提取物均在 MNTD 或 ½MNTD 时均显著抑制前脂肪细胞的成熟。
成熟脂肪细胞的形成受到抑制表明三角枫叶提取物可能具有潜在的抗肥胖作用。
