Zhang Hongliang, Leng Chaoliang, Ding Yushan, Zhai Hongyue, Li Zhen, Xiang Lirun, Zhang Wenli, Liu Chunxiao, Li Minhua, Chen Jiazeng, Bai Yun, Kan Yunchao, Yao Lunguang, Peng Jinmei, Wang Qian, Tang Yan-Dong, An Tongqing, Cai Xuehui, Tian Zhijun, Tong Guangzhi
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 678, Haping Road, Xiangfang District, Harbin, 150069, China.
Henan Key Laboratory of Insect Biology in Funiu Mountain, Henan Provincial Engineering Laboratory of Insects Bioreactor, China-UK-NYNU-RRes Joint Laboratory of Insect Biology, Nanyang Normal University, No. 1638, Wolong Road, Wolong District, Nanyang, 473061, China.
Arch Virol. 2019 Feb;164(2):401-411. doi: 10.1007/s00705-018-4080-7. Epub 2018 Oct 23.
Different strains of porcine reproductive and respiratory syndrome virus (PRRSV) have emerged and circulated in different regions of mainland China since 1996, particularly after 2006. In 2012, NADC30-like PRRSV was first isolated in Henan Province. By 2016, it had spread to most provinces in China. In the present study, the whole genomes (excluding the poly(A) tails) of 13 newly emerged NADC30-like PRRSV strains were sequenced and analyzed. Furthermore, the pathogenicity of SD53-1603, one of the 13 PRRSV strains, was assessed. Phylogenetic analysis showed that these 13 newly emerged NADC30-like PRRSV strains, together with some reference strains, formed a new subgroup (subgroup 5), characterized by a predicted 131-amino-acid deletion in the nonstructural protein (NSP) 2. However, low levels of whole-genome similarity and a wide variety of recombination patterns complicated the classification of the NADC30-like PRRSV isolates. Interestingly, almost all of the recombination breakpoints found in these 13 PRRSV isolates and other NADC30-like PRRSV isolates occurred in genes encoding NSPs and/or minor structural proteins. In addition, piglets infected with the newly emerged NADC30-like strain SD53-1603 displayed clear clinical respiratory symptoms and underwent typical pathological changes. The findings may be useful for elucidating the characteristics and epidemic status of NADC30-like PRRSV in China.
自1996年以来，不同毒株的猪繁殖与呼吸综合征病毒（PRRSV）在中国大陆不同地区出现并传播，特别是在2006年之后。2012年，类NADC30 PRRSV首次在河南省分离出来。到2016年，它已传播到中国的大多数省份。在本研究中，对13株新出现的类NADC30 PRRSV毒株的全基因组（不包括poly(A)尾）进行了测序和分析。此外，还评估了这13株PRRSV毒株之一的SD53-1603的致病性。系统发育分析表明，这13株新出现的类NADC30 PRRSV毒株与一些参考毒株一起形成了一个新的亚组（亚组5），其特征是在非结构蛋白（NSP）2中预测有131个氨基酸的缺失。然而，全基因组相似性水平较低以及多种重组模式使类NADC30 PRRSV分离株难以分类。有趣的是，在这13株PRRSV分离株和其他类NADC30 PRRSV分离株中发现的几乎所有重组断点都发生在编码NSP和/或次要结构蛋白的基因中。此外，感染新出现的类NADC30毒株SD53-1603的仔猪表现出明显的临床呼吸道症状，并出现典型的病理变化。这些发现可能有助于阐明中国类NADC30 PRRSV的特征和流行状况。
