Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan.
Department of Biochemistry and Biotechnology, University of Gujrat, Gujrat, 50700, Pakistan.
Daru. 2018 Dec;26(2):179-190. doi: 10.1007/s40199-018-0223-3. Epub 2018 Oct 23.
This study was aimed to evaluate the effect of Hedera nepalensis crude extract (HNC) and its isolated compound lupeol on antioxidant defence system, biochemical parameters and behavioural indices of Alzheimer disease generated in diabetic rats.
To evaluate the effect of the plant extract and lupeol, symptoms of Alzheimer and diabetes were induced in rats by STZ + AlCl treatment. Glucose level was measured with glucometer followed by antioxidant and biochemical assessment of the treated and untreated animals. Behavioural response of the rats was determined by Elevated Plus Maze (EPM) test and Morris Water Maze (MWM) test followed by determination of brain neurotransmitters by HPLC.
HNC significantly reduced blood glucose level in a time dependent manner and elevated liver function markers were significantly (P < 0.05) reinstated to normal levels. HNC showed increase in level of catalase (CAT), superoxide dismutase (SOD) and reduced glutathione (GSH). HPLC quantification revealed that HNC treatment led to significant (p < 0.001) elevation in the level of neurotransmitters (dopamine and serotonin) in the midbrain region as compared to Alzheimer control (AC) group. EPM and MWM test showed decrease in cognitive and memory impairment in a rat group treated with HNC as compared to AC group.
Overall, results showed that H. nepalensis has therapeutic potential for the treatment of diseases like Alzheimer and diabetes. Graphical abstract Therapeutic effect of Hedera nepalensis K. Koch and lupeol against STZ + AICI induced diabetic rats model.
本研究旨在评估尼泊尔常春藤粗提取物（HNC）及其分离化合物羽扇醇对糖尿病大鼠阿尔茨海默病模型抗氧化防御系统、生化参数和行为指标的影响。
通过 STZ+AlCl 处理诱导植物提取物和羽扇醇治疗的阿尔茨海默病和糖尿病症状，来评估植物提取物和羽扇醇的作用。用血糖仪测量血糖水平，然后对治疗和未治疗动物进行抗氧化和生化评估。通过高架十字迷宫（EPM）试验和水迷宫（MWM）试验测定大鼠的行为反应，然后通过 HPLC 测定脑神经递质。
HNC 能显著降低血糖水平，且具有时间依赖性，肝功能标志物也显著（P<0.05）恢复正常水平。HNC 增加了过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和还原型谷胱甘肽（GSH）的水平。HPLC 定量分析显示，与阿尔茨海默病对照组（AC）相比，HNC 治疗组中脑区神经递质（多巴胺和 5-羟色胺）水平显著升高（p<0.001）。EPM 和 MWM 试验表明，与 AC 组相比，HNC 治疗组大鼠的认知和记忆损伤程度降低。
总的来说，结果表明尼泊尔常春藤具有治疗阿尔茨海默病和糖尿病等疾病的潜在治疗作用。
