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纯化的E蛋白促进微小F质粒在体外的复制。

Replication of mini-F plasmid in vitro promoted by purified E protein.

作者信息

Muraiso K, Tokino T, Murotsu T, Matsubara K

出版信息

Mol Gen Genet. 1987 Mar;206(3):519-21. doi: 10.1007/BF00428895.

DOI:10.1007/BF00428895
PMID:3035347
Abstract

An in vitro system for replication of mini-F plasmid DNA was constructed. This system consists of an ammonium sulfate fraction II (Fuller et al. 1981) from Escherichia coli extract, exogenously added purified E protein encoded by mini-F plasmid, and mini-F DNA in a closed circular form. Experiments with this system showed that the 217 bp DNA region which contains the A + T rich cluster and the four 19 bp direct repeats responsible for incB incompatibility is essential for mini-F DNA replication.

摘要

构建了一个用于复制微型F质粒DNA的体外系统。该系统由来自大肠杆菌提取物的硫酸铵分级分离物II（富勒等人，1981年）、外源添加的由微型F质粒编码的纯化E蛋白以及闭环形式的微型F DNA组成。用该系统进行的实验表明，包含富含A + T的簇和负责IncB不相容性的四个19 bp直接重复序列的217 bp DNA区域对于微型F DNA复制至关重要。

相似文献

1
Replication of mini-F plasmid in vitro promoted by purified E protein.纯化的E蛋白促进微小F质粒在体外的复制。
Mol Gen Genet. 1987 Mar;206(3):519-21. doi: 10.1007/BF00428895.
2
Purification and properties of the mini-F plasmid-encoded E protein needed for autonomous replication control of the plasmid.用于质粒自主复制控制的微小F质粒编码E蛋白的纯化及特性
Proc Natl Acad Sci U S A. 1986 Jun;83(12):4109-13. doi: 10.1073/pnas.83.12.4109.
3
The repeated sequences (incB) preceding the protein E gene of plasmid mini-F are essential for replication.质粒mini-F的蛋白质E基因之前的重复序列（incB）对复制至关重要。
Mol Gen Genet. 1986 Jan;202(1):132-5. doi: 10.1007/BF00330529.
4
Mechanism of autonomous control of the Escherichia coli F plasmid: purification and characterization of the repE gene product.大肠杆菌F质粒自主控制机制：repE基因产物的纯化与特性分析
Nucleic Acids Res. 1988 Jan 25;16(2):413-24. doi: 10.1093/nar/16.2.413.
5
Nine unique repeating sequences in a region essential for replication and incompatibility of the mini-F plasmid.在微小F质粒复制和不相容性所必需的区域中的九个独特重复序列。
Gene. 1981 Nov;15(2-3):257-71. doi: 10.1016/0378-1119(81)90135-9.
6
Direct repeats of the F plasmid incC region express F incompatibility.F质粒incC区域的直接重复序列表达F不相容性。
Cell. 1981 Jun;24(3):687-94. doi: 10.1016/0092-8674(81)90095-7.
7
Complementation of replication-deficient deletion derivatives of plasmid mini-F.质粒mini-F复制缺陷型缺失衍生物的互补作用
J Bacteriol. 1981 Sep;147(3):736-43. doi: 10.1128/jb.147.3.736-743.1981.
8
Roles of Escherichia coli heat shock proteins DnaK, DnaJ and GrpE in mini-F plasmid replication.大肠杆菌热休克蛋白DnaK、DnaJ和GrpE在微型F质粒复制中的作用。
Mol Gen Genet. 1990 Jan;220(2):277-82. doi: 10.1007/BF00260494.
9
Regulation of DNA replication by iterons: an interaction between the ori2 and incC regions mediated by RepE-bound iterons inhibits DNA replication of mini-F plasmid in Escherichia coli.迭代子对DNA复制的调控：由结合RepE的迭代子介导的ori2和incC区域之间的相互作用抑制了大肠杆菌中mini-F质粒的DNA复制。
EMBO J. 1999 Jul 1;18(13):3856-67. doi: 10.1093/emboj/18.13.3856.
10
Replication of lambda dv plasmid in vitro promoted by purified lambda O and P proteins.纯化的λ O和P蛋白促进λ dv质粒的体外复制。
Proc Natl Acad Sci U S A. 1982 Dec;79(24):7639-43. doi: 10.1073/pnas.79.24.7639.

引用本文的文献

1
Replication initiator protein RepE of mini-F plasmid: functional differentiation between monomers (initiator) and dimers (autogenous repressor).微小F质粒的复制起始蛋白RepE：单体（起始蛋白）与二聚体（自体阻遏物）之间的功能分化
Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3839-43. doi: 10.1073/pnas.91.9.3839.
2
DNA-binding domain of the RepE initiator protein of mini-F plasmid: involvement of the carboxyl-terminal region.微小F质粒RepE起始蛋白的DNA结合结构域：羧基末端区域的作用
J Bacteriol. 1995 Apr;177(8):1994-2001. doi: 10.1128/jb.177.8.1994-2001.1995.
3
Site-specific proteolysis of mini-F plasmid replication protein RepE destroys initiator function and generates an incompatibility substance.

本文引用的文献

1
Replication and incompatibility of F and plasmids in the IncFI Group.F质粒及IncFI组中质粒的复制与不相容性
Plasmid. 1981 Jan;5(1):100-26. doi: 10.1016/0147-619x(81)90079-2.
2
Separation of the minimal replication region of the F plasmid into a replication origin segment and a trans-acting segment.F质粒最小复制区域分离为复制起始片段和反式作用片段。
Mol Gen Genet. 1982;186(3):372-7. doi: 10.1007/BF00729456.
3
Identification of the minimal essential region for the replication origin of miniF plasmid.微小F质粒复制起点最小必需区域的鉴定
微小F质粒复制蛋白RepE的位点特异性蛋白水解作用会破坏引发功能并产生一种不相容物质。
J Bacteriol. 1992 May;174(9):3004-10. doi: 10.1128/jb.174.9.3004-3010.1992.
Mol Gen Genet. 1984;196(2):373-8. doi: 10.1007/BF00328075.
4
An amber replication mutant of F plasmid mapped in the minimal replication region.F质粒的一个琥珀复制突变体定位在最小复制区域。
Mol Gen Genet. 1983;191(2):231-7. doi: 10.1007/BF00334819.
5
Role of nine repeating sequences of the mini-F genome for expression of F-specific incompatibility phenotype and copy number control.F 小基因组的九个重复序列在 F 特异性不相容表型表达和拷贝数控制中的作用。
J Bacteriol. 1983 Jul;155(1):337-44. doi: 10.1128/jb.155.1.337-344.1983.
6
An Mr 29000 protein is essential for mini-F maintenance in E. coli.一种分子量为29000的蛋白质对大肠杆菌中微型F质粒的维持至关重要。
Gene. 1982 Sep;19(2):173-8. doi: 10.1016/0378-1119(82)90003-8.
7
Enzymatic replication of the origin of the Escherichia coli chromosome.大肠杆菌染色体复制起点的酶促复制
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7370-4. doi: 10.1073/pnas.78.12.7370.
8
A review of mini-F plasmid maintenance.小型F质粒维持的综述。
Plasmid. 1985 Jul;14(1):1-16. doi: 10.1016/0147-619x(85)90027-7.
9
Purification and properties of the mini-F plasmid-encoded E protein needed for autonomous replication control of the plasmid.用于质粒自主复制控制的微小F质粒编码E蛋白的纯化及特性
Proc Natl Acad Sci U S A. 1986 Jun;83(12):4109-13. doi: 10.1073/pnas.83.12.4109.
10
Two functions of the E protein are key elements in the plasmid F replication control system.E蛋白的两种功能是质粒F复制控制系统中的关键要素。
J Bacteriol. 1985 Dec;164(3):1262-70. doi: 10.1128/jb.164.3.1262-1270.1985.