Norris J S, Brown P, Cohen J, Cornett L E, Kohler P O, MacLeod S L, Popovich K, Robey R B, Sifford M, Syms A J
Mol Cell Biochem. 1987 Mar;74(1):21-7. doi: 10.1007/BF00221909.
We have shown that glucocorticoids induce the appearance of beta 2-adrenergic receptors in membranes of the ductus deferens smooth muscle cell line (DDT1 MF-2). A concomitant increase in isoproterenol stimulated adenylate cyclase activity in the absence of exogenously applied GTP was observed as was a significantly increased (p less than 0.05) sensitivity of the adenylate cyclase system to exogenously applied GTP. However, no significant difference in the maximal velocity of adenylate cyclase between control and steroid treatment was measurable in the presence of sodium fluoride. Induction of beta 2-adrenergic receptors in DDT1 MF-2 cells is correlated with the presence of steroid receptors (androgen and glucocorticoid) in the cells since estrogens and progesterones had no effect on receptor levels. Finally, utilizing dense amino acid labeling of cells to measure old versus newly synthesized receptor sites by a density shift method, we have documented that glucocorticoid induction of beta 2-adrenergic receptors involves synthesis of new receptor protein.
我们已经证明，糖皮质激素可诱导输精管平滑肌细胞系（DDT1 MF-2）膜中β2-肾上腺素能受体的出现。在未施加外源性GTP的情况下，观察到异丙肾上腺素刺激的腺苷酸环化酶活性同时增加，并且腺苷酸环化酶系统对外源性施加的GTP的敏感性显著增加（p < 0.05）。然而，在存在氟化钠的情况下，对照和类固醇处理之间腺苷酸环化酶的最大速度没有可测量的显著差异。DDT1 MF-2细胞中β2-肾上腺素能受体的诱导与细胞中类固醇受体（雄激素和糖皮质激素）的存在相关，因为雌激素和孕激素对受体水平没有影响。最后，利用细胞的密集氨基酸标记，通过密度转移法测量旧的与新合成的受体位点，我们已经证明糖皮质激素诱导β2-肾上腺素能受体涉及新受体蛋白的合成。
