Immunoendocrinology, Division of Medical Biology, Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Hanzeplein 1, 9700, RB, Groningen, The Netherlands.
FrieslandCampina, Stationsplein 4, 3818, LE, Amersfoort, The Netherlands.
Mol Nutr Food Res. 2018 Dec;62(23):e1800716. doi: 10.1002/mnfr.201800716. Epub 2018 Nov 2.
Wheat hydrolysates are used in medical nutrition to provide undernourished patients a readily digestible protein source, for instance to recover from chemotherapy-induced intestinal mucosal inflammation. Since many hydrolysates of different sources can modulate the immune system, likely via Toll-like receptors (TLRs), it is hypothesized that also wheat hydrolysates might interact with TLR signaling, which could be a way to prevent intestinal inflammation and damage.
The capacity of three wheat hydrolysates to modulate immunity by interfering with TLR signaling is determined. All wheat hydrolysates have TLR modulating effects but only one has strong TLR2 inhibiting effects, attenuating both TLR2/1 and TLR2/6 signaling in a reporter cell system. This is likely induced by direct TLR2-ectodomain binding, as confirmed by ELISA. Furthermore, this TLR2 blocking hydrolysate reduces IL-6 production in human dendritic cells. Application of reversed-phase-ultra HPLC combined with MS reveals that the presence of peptide WQIPEQSR is associated with the observed TLR2 inhibiting capacity.
The study demonstrates TLR2-inhibiting capacities of a wheat hydrolysate. The findings provide a good start for further research to investigate whether this hydrolysate might contribute to the management of intestinal mucosal inflammation in cancer patients receiving chemotherapy.
小麦水解物被用于医学营养中，为营养不良的患者提供易消化的蛋白质来源，例如在化疗引起的肠道黏膜炎症后恢复。由于许多不同来源的水解物可以通过 Toll 样受体 (TLR) 调节免疫系统，因此推测小麦水解物也可能与 TLR 信号通路相互作用，这可能是预防肠道炎症和损伤的一种方式。
确定了三种小麦水解物通过干扰 TLR 信号通路调节免疫的能力。所有的小麦水解物都具有调节免疫的作用，但只有一种具有很强的 TLR2 抑制作用，在报告细胞系统中减弱 TLR2/1 和 TLR2/6 信号。这可能是由于直接与 TLR2 胞外结构域结合所致，ELISA 实验证实了这一点。此外，这种 TLR2 阻断水解物还可减少人树突状细胞中 IL-6 的产生。反相超高效液相色谱法与 MS 的联用揭示了肽 WQIPEQSR 的存在与观察到的 TLR2 抑制能力相关。
该研究证明了小麦水解物具有 TLR2 抑制作用。这些发现为进一步研究提供了良好的开端，以探讨这种水解物是否有助于管理接受化疗的癌症患者的肠道黏膜炎症。
