Reichart E, Huyen V N, Boerkmann P, Molon-Noblot S, Lecoffre C
Pathol Biol (Paris). 1987 Apr;35(4):362-6.
The endotracheal deposition of pancreatic porcine elastase (EPP) at 40 u X kg-1 body weight provokes a typical pulmonary emphysema (alveoli disruption) in rats. This emphysema is significant (p less than 0.001) when quantitatively compared to a placebo (100% increase of the mean linear intercept, ILM). The EPP-treated lungs are very heterogeneous and the size of the alveoli vary as much as 30% versus 19% in controls. The emphysema is more effective at a 80 u X kg-1 dose and the individual disparities are reduced (dose effect). When bacterial collagenase (200 u X kg-1 body weight) is added to EPP, the pulmonary abnormalities (disruptions, ILM) are in no way increased (no enzymatic synergy, repair by collagenesis?). In contrast, alveolar dilation is slightly reduced 8 weeks after enzyme administration (p less than 0.05): EPP is not altered in vitro by collagenase and despite several hypothesis, the moderating effect of collagenase in vivo still remains unexplained. This result suggests that the joint presence of several proteases is not necessarily an aggravating factor in the etiopathogenesis of emphysema.
以40单位/千克体重的剂量经气管内注入猪胰腺弹性蛋白酶（EPP）可在大鼠中引发典型的肺气肿（肺泡破裂）。与给予安慰剂相比，这种肺气肿具有显著性差异（p<0.001）（平均线性截距（ILM）增加了100%）。经EPP处理的肺组织异质性很强，肺泡大小的差异可达30%，而对照组为19%。80单位/千克体重的剂量引发的肺气肿更明显，个体差异减小（剂量效应）。当将细菌胶原酶（200单位/千克体重）与EPP联合使用时，肺部异常情况（破裂、ILM）并未增加（无酶协同作用，通过胶原生成进行修复？）。相反，酶给药8周后肺泡扩张略有减小（p<0.05）：胶原酶在体外不会改变EPP，尽管有多种假设，但胶原酶在体内的调节作用仍无法解释。该结果表明，多种蛋白酶共同存在不一定是肺气肿发病机制中的加重因素。
