Fujita A, Hattori M, Takenaka O, Sakaki Y
Nucleic Acids Res. 1987 May 26;15(10):4007-20. doi: 10.1093/nar/15.10.4007.
We previously reported that some L1 family (KpnI family) members are closely associated with the Alu family sequence. To understand the details of the L1-Alu association, the structure of a L1-Alu unit downstream from the beta-globin gene was compared between human and primates. The results revealed that the L1-Alu-associated sequence was formed by the insertion of the L1 sequence, T beta G41, into the 3' poly A tract of the preexisting Alu family sequence. It was estimated that the T beta G41 sequence was inserted after the divergence of Old World monkeys and hominoids and before the divergence of orang-utan and common ancestor of other higher hominoids. From the calculation of the mutation rates of L1 sequences, it was suggested that the T beta G41 was derived from an active L1 sequence which was able to encode reverse transcriptase-related protein.
我们先前报道过，一些L1家族（KpnI家族）成员与Alu家族序列密切相关。为了解L1与Alu关联的细节，我们比较了人类和灵长类动物β-珠蛋白基因下游L1-Alu单元的结构。结果显示，L1-Alu相关序列是由L1序列TβG41插入到预先存在的Alu家族序列的3'多聚腺苷酸区域形成的。据估计，TβG41序列是在旧世界猴和类人猿分化之后、猩猩与其他高等类人猿共同祖先分化之前插入的。通过计算L1序列的突变率，表明TβG41源自一个能够编码逆转录酶相关蛋白的活跃L1序列。
