Fornari Gheniffer, Gomes Renata Rodrigues, Degenhardt-Goldbach Juliana, Dos Santos Suelen Silvana, de Almeida Sandro Rogério, Dos Santos Germana Davila, Muro Marisol Dominguez, Bona Cleusa, Scola Rosana Herminia, Trindade Edvaldo S, Bini Israel Henrique, Ferreira-Maba Lisandra Santos, Kestring Daiane Rigoni, do Nascimento Mariana Machado Fidelis, Lima Bruna Jacomel Favoreto de Souza, Voidaleski Morgana F, Steinmacher Douglas André, Soley Bruna da Silva, Deng Shuwen, Bocca Anamelia Lorenzetti, da Silva Moises B, Salgado Claudio G, de Azevedo Conceição Maria Pedroso E Silva, Vicente Vania Aparecida, de Hoog Sybren
Microbiology, Parasitology and Pathology Post-graduation Program, Department of Basic Pathology, Federal University of Paraná, Curitiba, Brazil.
Embrapa Forestry, Brazilian Agricultural Research Corporation (EMBRAPA), Colombo, Brazil.
Front Microbiol. 2018 Oct 9;9:2211. doi: 10.3389/fmicb.2018.02211. eCollection 2018.
The fungal genus comprises etiological agents of human chromoblastomycosis, a chronic implantation skin disease. The current hypothesis is that patients acquire the infection through an injury from plant material. The present study aimed to evaluate a model of infection in plant and animal hosts to understand the parameters of trans-kingdom pathogenicity. Clinical strains of causative agents of chromoblastomycosis ( and ) were compared with a strain of isolated from a living plant. The clinical strains of and remained concentrated near the epidermis, whereas colonized deeper plant tissues, resembling an endophytic behavior. In an invertebrate infection model with larvae of a beetle, exhibited the lowest survival rates. However, produced dark, spherical to ovoidal cells that resembled muriform cells, the invasive form of human chromoblastomycosis confirming the role of muriform cells as a pathogenic adaptation in animal tissues. An immunologic assay in BALB/c mice demonstrated the high virulence of saprobic species in animal models was subsequently controlled via host higher immune response.
该真菌属包含人类着色芽生菌病的病原体，这是一种慢性植入性皮肤病。目前的假说是，患者通过植物材料造成的损伤而感染。本研究旨在评估植物和动物宿主中的感染模型，以了解跨界致病性的参数。将着色芽生菌病病原体（ 和 ）的临床菌株与从活植物中分离出的 菌株进行比较。 和 的临床菌株仍集中在表皮附近，而 则定殖于更深的植物组织中，类似于内生菌行为。在甲虫幼虫的无脊椎动物感染模型中， 表现出最低的存活率。然而， 产生了深色的、球形至卵形的细胞，类似于砖壁状细胞，这是人类着色芽生菌病的侵袭形式，证实了砖壁状细胞作为动物组织中致病适应性的作用。在BALB/c小鼠中进行的免疫测定表明，腐生菌在动物模型中的高毒力随后通过宿主更高的免疫反应得到控制。
